Bifunctional chimeric protein of endoxylanase/arabinofuranosidase for agro-industrial wastes hydrolysis and xylooligosaccharides production

• Grant number: 20/02871-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): March 01, 2021
• Effective date (End): September 30, 2024
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Principal Investigator: Rosana Goldbeck Coelho
• Grantee: Manoela Martins
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 22/04557-9 - Improving activity and thermostability of GH 62 a-L-arabinofuranosidase by directed evolution, BE.EP.DR

Abstract

Xylo-oligosaccharides have prebiotic activity, favoring intestinal function, immunological action and other health benefits. The production of these oligosaccharides can be carried out by enzymatic hydrolysis of lignocellulosic material, a selective, controlled and sustainable method. Genetic engineering tools allow the fusion of enzymes produced by different microorganisms, which have the potential to produce XOS, making it possible to obtain a stable bifunctional chimera capable of efficiently hydrolyzing the hemicellulosic fraction of agro-industrial waste, while reducing the production costs of enzymes. The present work aims the cloning and constitutive expression of arabinofuranosidase gene of A. fumigatus in Pichia pastoris using the pGAP vector, and later fusion with C. flavescens endoxylanase to obtain bifunctional chimeric protein. Hydrolysis profiles and conditions, synergy, specificity and structure of the obtained chimera will be investigated. The hydrolysis products (xylo-oligosaccharides) of lignocellulosic material from agro-industrial residues of corn, orange and soy husks, using the chimeric protein obtained will be quantified, purified and evaluated for antioxidant, prebiotic and antiproliferative activity. The presented project is associated to the FAPESP 2019/08542-3 Process (Enzymatic biotechnology for the production of xylooligosaccharides from lignocellulosic agroindustrial residues aiming at the production of symbiotics), in which we are inserted. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARTINS, MANOELA; SILVA, MARCOS FELLIPE; DINAMARCO, TAISA MAGNANI; GOLDBECK, ROSANA. Novel bi-functional thermostable chimeric enzyme for feasible xylo-oligosaccharides production from agro-industrial wastes. Process Biochemistry, v. 122, p. 10-pg., 2022-11-01. (20/02871-2, 19/08542-3)

MARTINS, MANOELA; TRAMONTINA, ROBSON; SQUINA, FABIO MARCIO; DINAMARCO, TAISA MAGNANI; GOLDBECK, ROSANA. Synergism for xylo-oligosaccharides, p-coumaric and ferulic acid production, and thermostability modulation of GH 62 alpha-L-arabinofuranosidase. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 44, p. 11-pg., 2022-08-26. (20/02871-2, 15/50590-4, 19/08542-3)

MARTINS, MANOELA; SGANZERLA, WILLIAM GUSTAVO; FORSTER-CARNEIRO, TANIA; GOLDBECK, ROSANA. Recent advances in xylo-oligosaccharides production and applications: A comprehensive review and bibliometric analysis. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 47, p. 16-pg., 2023-01-09. (19/26925-7, 19/08542-3, 18/14938-4, 20/02871-2)