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Dietary supplementation of amino acids on metabolism and growth performance of growing pigs housed under two different sanitary housing conditions

Grant number: 19/20150-3
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2021
Effective date (End): July 31, 2024
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
Principal Investigator:Luciano Hauschild
Grantee:Graziela Alves da Cunha Valini
Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Associated research grant:18/15559-7 - Precision nutrition for sanitary challenged pigs reared in hot climate areas, AP.JP2
Associated scholarship(s):22/02152-1 - Effect of amino acid supplementation and initial body protein on performance, body composition, and metabolism of growing pigs raised under sanitary challenge, BE.EP.DR


The objective of the project is to evaluate the effect of dietary supplementation of Amino Acids (AA) on the metabolism and performance of pigs housed under poor and good housing conditions. A total of 120 pigs, castrated males, an average body weight of 15 kg, will be randomly allocated in one of eight treatments in a 2 x 2 x 2 factorial scheme: two sanitary conditions - with or without sanitary challenged with Salmonella typhimurium (ST) (oral inoculation at 108 CFU dosage); two diets - control (CON) and enriched diet (ENR) with 20% increase in AA:Lys ratio (methionine, threonine, and tryptophan) and two moments of supplementation - before ST challenge (preventive supplementation) or after ST challenge (curative supplementation), during a 49 days experimental period. An automated feeding system (automated intelligent precision feeder) will record the feed intake and feed behavior (feeder occupation, feeding time per meal, and feed intake per meal). Weekly weightings will be used to evaluate growth performance. The body composition (lean, fat, and bone deposition) and microbiome characterization will be measured by Dual-energy X-ray Absorptiometry (DXA) and new generation sequencing methods, respectively. Finally, metabolic (creatinine, urea, and total amino acids) and immunological parameters (haptoglobin, lymphocyte count, immunoglobulins, diamine oxidase, and D-lactate) will be evaluated as well. The achievements of this study will improve the development of nutritional strategies to mitigate the effects of sanitary challenges on the metabolism, performance, and well-being of growing pigs. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ALVES DA CUNHA VALINI, GRAZIELA; RIGHETTI ARNAUT, PEDRO; FRANCA, ISMAEL; TREVISAN ORTIZ, MANOELA; KARPEGGIANE DE OLIVEIRA, MARLLON JOSE; BRANDAO MELO, ANTONIO DIEGO; ALVES MARCAL, DANILO; REIS FURTADO CAMPOS, PAULO HENRIQUE; KHUN HTOO, JOHN; GASTMANN BRAND, HENRIQUE; et al. Increased dietary Trp, Thr, and Met supplementation improves growth performance and protein deposition of salmonella-challenged growing pigs under poor housing conditions. JOURNAL OF ANIMAL SCIENCE, v. 101, p. 12-pg., . (19/20150-3, 18/15559-7)

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