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Extracellular matrix disruption and the use of a curcumin nanoparticle to potentiate the inactivation of Staphylococcus aureus biofilms

Grant number: 21/01324-0
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): April 01, 2021
Effective date (End): March 31, 2025
Field of knowledge:Biological Sciences - Biophysics - Radiology and Photobiology
Principal Investigator:Cristina Kurachi
Grantee:Fernanda Alves Dias de Sousa
Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Associated research grant:13/07276-1 - CEPOF - Optics and Photonic Research Center, AP.CEPID


Antimicrobial Photodynamic (aPDT) and Sonodynamic (SDT) therapies have been investigated as alternative methods for inactivating microorganisms. These treatments are based on the application of a sensitizer, which is activated by a light (aPDT) or an ultrasound (SDT), and are capable of generating reactive oxygen species that are lethal to microorganisms. However, the combination of both treatments, called Sonophotodynamic Therapy (SPDT), has proven to be more effective than the isolated application of the treatments. In addition, the use of nanoparticle sensitizer allows it to cross biological barriers, facilitating its delivery into the microbial cells. Thus, this study aims to compare the effectiveness of aPDT, SDT and SPDT when mediated by conventional synthetic curcumin or a curcumin nanoparticle, against Staphylococcus aureus biofilms. In addition to the effectiveness of the treatments, the capacity of these therapies to disrupt biofilms will be also investigated. For this, the components of the extracellular matrix will be measured, the Raman spectroscopy technique will be applied and the biofilms will be observed in a laser confocal microscope. In addition, the reactive oxygen species produced by each therapy will be quantified and the photodegradation of both sensitizers will be carried out. The results will be analyzed statistically by appropriate tests. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BUZZA, HILDE HARB; ALVES, FERNANDA; TOME, ANA JULIA BARBOSA; CHEN, JUAN; KASSAB, GIULIA; BU, JIACHUAN; BAGNATO, VANDERLEI SALVADOR; ZHENG, GANG; KURACHI, CRISTINA. Porphyrin nanoemulsion for antimicrobial photodynamic therapy: effective delivery to inactivate biofilm-related infections. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v. 119, n. 46, p. 9-pg., . (21/01324-0, 18/18188-0, 16/14033-6, 13/07276-1, 14/50857-8)
ALVES, FERNANDA; STRINGASCI, MIRIAN D.; REQUENA, MICHELLE B.; BLANCO, KATE C.; DIAS, LUCAS D.; CORREA, THAILA Q.; BAGNATO, VANDERLEI S.. Randomized and Controlled Clinical Studies on Antibacterial Photodynamic Therapy: An Overview. PHOTONICS, v. 9, n. 5, p. 36-pg., . (21/09952-0, 19/12694-3, 13/07276-1, 19/13569-8, 21/01324-0)
DIAS, LUCAS D.; ALVES, FERNANDA; BUZZA, HILDE H.; BAGNATO, VANDERLEI S.. Photodisinfection of material surfaces and bacterial skin infections by a detergent loaded with curcumin. Photodiagnosis and Photodynamic Therapy, v. 39, p. 7-pg., . (18/00106-7, 19/13569-8, 14/50857-8, 21/01324-0, 13/07276-1)
SILVA E CARVALHO, IAGO; PRATAVIEIRA, SEBASTIAO; BAGNATO, VANDERLEI SALVADOR; ALVES, FERNANDA. Sonophotodynamic inactivation of Pseudomonas aeruginosa biofilm mediated by curcumin. BIOFOULING, v. 39, n. 6, p. 11-pg., . (13/07276-1, 21/01324-0, 14/50857-8)

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