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Extracellular matrix disruption and the use of a curcumin nanoparticle to potentiate the inactivation of Staphylococcus aureus biofilms

Grant number: 21/01324-0
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): April 01, 2021
Effective date (End): March 31, 2023
Field of knowledge:Biological Sciences - Biophysics - Radiology and Photobiology
Principal researcher:Cristina Kurachi
Grantee:Fernanda Alves Dias de Sousa
Home Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Associated research grant:13/07276-1 - CEPOF - Optics and Photonic Research Center, AP.CEPID

Abstract

Antimicrobial Photodynamic (aPDT) and Sonodynamic (SDT) therapies have been investigated as alternative methods for inactivating microorganisms. These treatments are based on the application of a sensitizer, which is activated by a light (aPDT) or an ultrasound (SDT), and are capable of generating reactive oxygen species that are lethal to microorganisms. However, the combination of both treatments, called Sonophotodynamic Therapy (SPDT), has proven to be more effective than the isolated application of the treatments. In addition, the use of nanoparticle sensitizer allows it to cross biological barriers, facilitating its delivery into the microbial cells. Thus, this study aims to compare the effectiveness of aPDT, SDT and SPDT when mediated by conventional synthetic curcumin or a curcumin nanoparticle, against Staphylococcus aureus biofilms. In addition to the effectiveness of the treatments, the capacity of these therapies to disrupt biofilms will be also investigated. For this, the components of the extracellular matrix will be measured, the Raman spectroscopy technique will be applied and the biofilms will be observed in a laser confocal microscope. In addition, the reactive oxygen species produced by each therapy will be quantified and the photodegradation of both sensitizers will be carried out. The results will be analyzed statistically by appropriate tests. (AU)

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