Evaluation of the influence of the light on the expression of hydrolytic enzymes for the bioprospecting of fungi of the genus Trichoderma with hydrolytic potential

• Grant number: 20/13420-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: July 01, 2021
• End date: August 31, 2024
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Anete Pereira de Souza
• Grantee: Rafaela Rossi Rosolen
• Host Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Species in the filamentous ascomycete genus Trichoderma play an important role in producing and efficiently secreting carbohydrate-active enzyme (CAZyme), which are utilized industrially for the hydrolysis of plant biomass for the subsequent production of biofuels and high-value biochemicals. The expression of CAZyme-encoding genes is regulated by the action of several Transcription Factors (TFs) and dependent on the carbon source available in the extracellular medium. Additionally, studies have shown that abiotic factors, such as light, play a considerable role in the regulation of plant cell wall degrading enzymes. Therefore, the elucidation of the genetic mechanisms of the light in the expression of hydrolytic enzymes in different strains is necessary and can assist the bioprospecting of potentially hydrolytic fungi of the genus Trichoderma. Thus, by investigating the structure of the genome and the level of expression of target genes in light and dark conditions, we aim to study the influence of light on the expression of hydrolytic enzymes in strains of the genus Trichoderma. Exploiting of light-dependent effects will be crucial to generate strategies for the selection and improvement of Trichoderma species, especially in the production of enzymatic cocktails used in the saccharification of lignocellulosic substrates. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROSOLEN, RAFAELA ROSSI; AONO, ALEXANDRE HILD; ALMEIDA, DEBORAH AIRES; FERREIRA FILHO, JAIRE ALVES; HORTA, MARIA AUGUSTA CRIVELENTE; DE SOUZA, ANETE PEREIRA. Network Analysis Reveals Different Cellulose Degradation Strategies Across Trichoderma harzianum Strains Associated With XYR1 and CRE1. FRONTIERS IN GENETICS, v. 13, p. 19-pg., 2022-02-24. (18/19660-4, 20/13420-1, 15/09202-0, 19/03232-6)

FERREIRA FILHO, JAIRE A.; ROSOLEN, RAFAELA R.; ALMEIDA, DEBORAH A.; DE AZEVEDO, PAULO HENRIQUE C.; MOTTA, MARIA LORENZA L.; AONO, ALEXANDRE H.; DOS SANTOS, CLELTON A.; HORTA, MARIA AUGUSTA C.; DE SOUZA, ANETE P.. Trends in biological data integration for the selection of enzymes and transcription factors related to cellulose and hemicellulose degradation in fungi. 3 BIOTECH, v. 11, n. 11, NOV 2021. (15/09202-0, 19/03232-6, 20/13420-1, 18/19660-4, 20/10536-9)

ROSOLEN, RAFAELA ROSSI; HORTA, MARIA AUGUSTA CRIVELENTE; DE AZEVEDO, PAULO HENRIQUE CAMPITELI; DA SILVA, CARLA CRISTINA; SFORCA, DANILO AUGUSTO; GOLDMAN, GUSTAVO HENRIQUE; DE SOUZA, ANETE PEREIRA. Whole-genome sequencing and comparative genomic analysis of potential biotechnological strains of Trichoderma harzianum, Trichoderma atroviride, and Trichoderma reesei. Molecular Genetics and Genomics, v. 298, n. 3, p. 20-pg., 2023-04-05. (15/09202-0, 18/19660-4, 20/13420-1, 20/10536-9)