Genetic transformation of maize and Arabidopsis thaliana for functional evaluation of genes aiming at tolerance to abiotic stresses

• Grant number: 21/08486-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): September 01, 2021
• Effective date (End): August 31, 2024
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Paulo Arruda
• Grantee: Alessandra Koltun
• Host Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Host Company: Universidade Estadual de Campinas (UNICAMP). Centro de Biologia Molecular e Engenharia Genética (CBMEG)
• Associated research grant: 16/23218-0 - The Genomics for Climate Change Research Center, AP.PCPE
• Associated scholarship(s): 23/11557-8 - Enhancing efficiency of tropical maize genome transformation and editing through leaf explants and morphogenic genes, BE.EP.PD

Abstract

Global climate change poses a major threat to agricultural production and food security around the world, especially in the tropics. This proposal for a postdoctoral fellowship project aims to genetically transform maize and Arabidopsis thaliana to interrogate the function of abiotic-stress-related genes. The project involves the cultivation, optimization of transformation protocols, and evaluation of the role played by genes discovered in the GCCRC pipeline, mainly the ones related to mitochondrial metabolism. As for maize transformation, the grantee will work in collaboration with the transformation team to optimize the process. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

JOSÉ HERNANDES-LOPES; JULIANA ERIKA DE CARVALHO TEIXEIRA YASSITEPE; ALESSANDRA KOLTUN; LAURENS PAUWELS; VIVIANE CRISTINA HEINZEN DA SILVA; RICARDO AUGUSTO DANTE; ISABEL RODRIGUES GERHARDT; PAULO ARRUDA. Genome editing in maize: Toward improving complex traits in a global crop. GENETICS AND MOLECULAR BIOLOGY, v. 46, n. 1, 2023-03-03. (16/23218-0, 18/06442-9, 21/08486-6)

BARRETO, PEDRO; KOLTUN, ALESSANDRA; NONATO, JULIANA; YASSITEPE, JULIANA; MAIA, IVAN DE GODOY; ARRUDA, PAULO. Metabolism and Signaling of Plant Mitochondria in Adaptation to Environmental Stresses. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 23, n. 19, p. 34-pg., 2022-10-01. (21/01003-0, 16/23218-0, 21/08486-6)