Additive-manufactured (3D printed) electrochemical devices for the diagnosis of viral diseases

• Grant number: 21/07989-4
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: February 01, 2022
• End date: January 31, 2023
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Juliano Alves Bonacin
• Grantee: Cristiane Kalinke
• Supervisor: Craig E. Banks
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Institution abroad: Manchester Metropolitan University (MMU), England
• Associated to the scholarship: 19/00473-2 - Development of 3-dimensional (3D) printed electrochemical biosensors with PLA polymer and graphene for the determination of biomolecules and diagnosis of diseases, BP.PD

Abstract

The use of additive manufacturing or 3D printing allows the development of simple and low-cost lab-made electrochemical devices, with the possibility of modulation in different shapes and miniaturization, leading to the construction of versatile and portable devices. In this sense, the use of conductive filaments has become extremely advantageous for the obtaining of electrochemical sensors. However, the sensor characteristics can be improved by making filaments in the laboratory, which allows its modification with a greater amount of conductive material. This leads to the development of new materials with high electron transfer and conductivity, lower resistivity, and the ability to interact and/or immobilize other species. This project aims to obtain new conductive materials, such as polymeric filaments or resins, for the development of 3D printed electrochemical sensors. Parallel to this, a complete and fully 3D printed electrochemical cell will be developed exploring different printing materials and techniques. The immobilization of biological materials on the electrode surface will allow the development of a biosensor, which will be applied for the detection and diagnosis of emerging viral diseases, such as yellow fever, dengue, and COVID-19, in biological samples. Therefore, it is expected to obtain lab-made, portable, and patentable electrochemical devices for the early diagnosis of viral diseases. (AU)

Scientific publications

(12)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CRAPNELL, ROBERT D.; SIGLEY, EVELYN; WILLIAMS, RHYS J.; BRINE, TOM; GARCIA-MIRANDA FERRARI, ALEJANDRO; KALINKE, CRISTIANE; JANEGITZ, BRUNO C.; BONACIN, JULIANO A.; BANKS, CRAIG E.. Circular Economy Electrochemistry: Recycling Old Mixed Material Additively Manufactured Sensors into New Electroanalytical Sensing Platforms. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 11, n. 24, p. 11-pg., 2023-06-07. (17/21097-3, 21/07989-4, 19/00473-2, 13/22127-2)

KALINKE, CRISTIANE; DE OLIVEIRA, PAULO ROBERTO; BANKS, CRAIG E.; JANEGITZ, BRUNO CAMPOS; BONACIN, JULIANO ALVES. 3D-printed immunosensor for the diagnosis of Parkinson?s disease. SENSORS AND ACTUATORS B-CHEMICAL, v. 381, p. 8-pg., 2023-01-27. (19/01844-4, 19/00473-2, 17/21097-3, 21/07989-4, 13/22127-2)

JANEGITZ, BRUNO C.; CRAPNELL, ROBERT D.; DE OLIVEIRA, PAULO ROBERTO; KALINKE, CRISTIANE; WHITTINGHAM, MATTHEW J.; FERRARI, ALEJANDRO GARCIA-MIRANDA; BANKS, CRAIG E.. Novel Additive Manufactured Multielectrode Electrochemical Cell with Honeycomb Inspired Design for the Detection of Methyl Parathion in Honey Samples. ACS MEASUREMENT SCIENCE AU, v. 3, n. 3, p. 9-pg., 2023-06-21. (17/21097-3, 21/07989-4, 22/01601-7, 19/00473-2)

DE OLIVEIRA, PAULO ROBERTO; CRAPNELL, ROBERT D.; FERRARI, ALEJANDRO GARCIA -MIRANDA; WUAMPRAKHON, PHATSAWIT; HURST, NICHOLAS J.; DEMPSEY-HIBBERT, NINA C.; SAWANGPHRUK, MONTREE; JANEGITZ, BRUNO CAMPOS; BANKS, CRAIG E.. Low-cost, facile droplet modification of screen-printed arrays for internally validated electrochemical detection of serum procalcitonin. BIOSENSORS & BIOELECTRONICS, v. 228, p. 9-pg., 2023-03-14. (17/21097-3, 21/07989-4, 22/01601-7, 19/00473-2)

CRAPNELL, ROBERT D.; ARANTES, IANA V. S.; WHITTINGHAM, MATTHEW J.; SIGLEY, EVELYN; KALINKE, CRISTIANE; JANEGITZ, BRUNO C.; BONACIN, JULIANO A.; PAIXAO, THIAGO R. L. C.; BANKS, CRAIG E.. Utilising bio-based plasticiser castor oil and recycled PLA for the production of conductive additive manufacturing feedstock and detection of bisphenol A. GREEN CHEMISTRY, v. 25, n. 14, p. 10-pg., 2023-06-22. (19/00473-2, 22/07552-8, 17/21097-3, 18/08782-1, 21/07989-4, 19/15065-7, 13/22127-2)

BLASQUES, RODRIGO VIEIRA; DE OLIVEIRA, PAULO ROBERTO; KALINKE, CRISTIANE; BRAZACA, LAIS CANNIATTI; CRAPNELL, ROBERT D. D.; BONACIN, JULIANO ALVES; BANKS, CRAIG E. E.; JANEGITZ, BRUNO CAMPOS. Flexible Label-Free Platinum and Bio-PET-Based Immunosensor for the Detection of SARS-CoV-2. BIOSENSORS-BASEL, v. 13, n. 2, p. 19-pg., 2023-02-01. (19/00473-2, 17/21097-3, 18/19750-3, 19/23177-0, 21/07989-4, 13/22127-2)

STEFANO, JESSICA SANTOS; SILVA, LUIZ RICARDO GUTERRES E; KALINKE, CRISTIANE; DE OLIVEIRA, PAULO ROBERTO; CRAPNELL, ROBERT D.; BRAZACA, LAIS CANNIATTI; BONACIN, JULIANO ALVES; CAMPUZANO, SUSANA; BANKS, CRAIG E.; JANEGITZ, BRUNO CAMPOS. Human monkeypox virus: Detection methods and perspectives for diagnostics. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 167, p. 18-pg., 2023-10-01. (17/21097-3, 21/07989-4, 22/06145-0, 13/22127-2)

VON ZUBEN, THEODORA WROBEL; KALINKE, CRISTIANE; JANEGITZ, BRUNO CAMPOS; SALLES JR, AIRTON GONCALVES; BONACIN, JULIANO ALVES. 3D-printed amperometric sensor for the detection of ethanol in saliva. Electroanalysis, v. N/A, p. 11-pg., 2023-09-29. (21/07989-4, 19/21506-6, 13/22127-2, 19/00473-2)

SIGLEY, EVELYN; KALINKE, CRISTIANE; CRAPNELL, ROBERT D.; WHITTINGHAM, MATTHEW J.; WILLIAMS, RHYS J.; KEEFE, EDMUND M.; JANEGITZ, BRUNO CAMPOS; BONACIN, JULIANO ALVES; BANKS, CRAIG E.. Circular Economy Electrochemistry: Creating Additive Manufacturing Feedstocks for Caffeine Detection from Post- Industrial Coffee Pod Waste. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 11, n. 7, p. 11-pg., 2023-02-20. (19/01844-4, 19/00473-2, 17/21097-3, 21/07989-4, 13/22127-2)

OLIVEIRA, PAULO ROBERTO; GUTERRES E SILVA, LUIZ RICARDO; KALINKE, CRISTIANE; FERRARI, ALEJANDRO GARCIA-MIRANDA; PRAKASH, JAI; WEN, YANGPING; NOCELLI, ROBERTA CORNELIO FERREIRA; BONACIN, JULIANO ALVES; BANKS, CRAIG E.; JANEGITZ, BRUNO CAMPOS. Conductive Biofilm Propolis-Based: Electrochemical Determination of Hydroxymethylfurfural in Honey. FOOD ANALYTICAL METHODS, v. N/A, p. 10-pg., 2023-07-31. (19/01844-4, 19/00473-2, 17/21097-3, 22/01601-7, 21/07989-4, 13/22127-2)

DA SILVA, VINICIUS A. O. P.; STEFANO, JESSICA S.; KALINKE, CRISTIANE; BONACIN, JULIANO. A. A.; JANEGITZ, BRUNO. C. C.. Additive Manufacturing Sensor for Stress Biomarker Detection. CHEMOSENSORS, v. 11, n. 5, p. 14-pg., 2023-05-20. (22/06145-0, 19/00473-2, 17/21097-3, 21/03179-8, 21/07989-4)

KALINKE, CRISTIANE; CRAPNELL, ROBERT D.; SIGLEY, EVELYN; WHITTINGHAM, MATTHEW J.; DE OLIVEIRA, PAULO R.; BRAZACA, LAIS C.; JANEGITZ, BRUNO C.; BONACIN, JULIANO A.; BANKS, CRAIG E.. Recycled additive manufacturing feedstocks with carboxylated multi-walled carbon nanotubes toward the detection of yellow fever virus cDNA. CHEMICAL ENGINEERING JOURNAL, v. 467, p. 10-pg., 2023-05-19. (19/01844-4, 19/00473-2, 17/21097-3, 21/07989-4, 13/22127-2)