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Senna spp. extracts fractionation: phytochemical study and evaluation as photosensitizers on Candida albicans and Staphylococcus aureus monotypic and heterotypic biofilms

Grant number: 20/16330-3
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): October 01, 2021
Effective date (End): February 28, 2023
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Fernanda Lourenção Brighenti
Grantee:Elis Rodrigues Oliveira Barbosa
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil


The photodynamic therapy is an alternative for conventional infections treatments given its low probability to cause microbiology resistance. The purpose of this project is to carry out a phytochemical study of Senna spp. fractions and test their in vitro potential as monotherapy in antimicrobial photodynamic therapy on Candida albicans and Staphylococcus aureus monotypic and heterotypic biofilms. Fractionation and chemical characterization of Senna macranthera, Senna reticulata and Senna splendida extracts will be performed. Biofilms will be formed for 48 h and then submitted to antimicrobial photodynamic therapy with 0,50 mg/mL of the extracts fractions. Overall nine groups will be studied: G1- negative control, G2- S. splendida fractions, G3- S. reticulata fractions, G4- S. macranthera fractions, G5- light, G6- photodynamic therapy with S. splendida fractions, G7- photodynamic therapy with S. reticulata fractions, G8- photodynamic therapy with S. macranthera fractions and G9- medium control. Color change evaluation of bovine teeth, internalization test to analyze the penetration of extracts into biofilms and cytotoxicity tests will also be performed. For statistical analysis, the SPSS 20.0 software will be used and a 5% significance level established.

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BARBOSA, Elis Rodrigues Oliveira. Evaluation of antimicrobial photodynamic therapy application for control and treatment of infections involving Candida spp. and Staphylococcus aureus. 2023. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.

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