Treatment of pharyngotonsillitis with photodynamic action: investigation of basic mechanisms

• Grant number: 21/09952-0
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: January 17, 2022
• End date: January 16, 2023
• Field of knowledge: Biological Sciences - Biophysics - Radiology and Photobiology
• Principal Investigator: Vanderlei Salvador Bagnato
• Grantee: Kate Cristina Blanco
• Supervisor: Vladislav Yakovlev
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Institution abroad: Texas A&M University, United States
• Associated to the scholarship: 19/12694-3 - Treatment of pharyngotonsillitis with photodynamic action, BP.PD

Abstract

This is a research proposal that is being directed to the Research Scholarship Abroad program (BEPE) - FAPESP and proposes a partnership between the University A&M Texas (USA) and the University of São Paulo (Brazil) in biomedical research for combating multiresistant bacteria. For this, we intend to explore new scientific ideas, tools and technologies that have the potential to significantly impact the therapy of infections caused by multi-resistant bacteria, which is common to the needs of biomedical research in the US and Brazil. A significant number of treatment failures for streptococcal pharyngotonsillitis (FT) are described in the scientific literature relating the chronicity and recurrent characteristics of some forms of the infection. Internalization and colonization of group A Streptococcus (GAS) in tonsillar epithelial cells by multiresistant strains and biofilms in inflamed tonsil tissue and crypts have been identified as potential causes of antibiotic treatment failures (ATB). A clinical study is being carried out with photodynamic therapy (PDT) for the treatment of acute FT and now a step forward must be taken, providing an understanding to alleviate the complex problem of multidrug-resistant bacteria in these types of infections. Breaking antimicrobial resistance by PDT provides evidence to suggest that antibiotics may increase their effectiveness against bacteria. The nature of this interaction is unclear and complicates efforts to integrate the oxidative stress of PDT into the treatment of infections. This study will aim to determine whether these treatments are truly synergistic or simply orthogonal based on the investigation of antimicrobial resistance breakdown by PDT. The proposed research involves an intense activity in this sense by two research institutions that have already been collaborating in scientific terms. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOARES, JENNIFER M.; GUIMARAES, FRANCISCO E. G.; YAKOVLEV, VLADISLAV V.; BAGNATO, VANDERLEI S.; BLANCO, KATE C.. Physicochemical mechanisms of bacterial response in the photodynamic potentiation of antibiotic effects. SCIENTIFIC REPORTS, v. 12, n. 1, p. 11-pg., 2022-12-07. (19/12694-3, 14/50857-8, 13/07276-1, 21/09952-0)

WILLIS, JACE A.; CHEBURKANOV, VSEVOLOD; CHEN, SHAORONG; SOARES, JENNIFER M.; KASSAB, GIULIA; BLANCO, KATE C.; BAGNATO, VANDERLEI S.; DE FIGUEIREDO, PAUL; YAKOVLEV, VLADISLAV V.. Breaking down antibiotic resistance in methicillin-resistant Staphylococcus aureus: Combining antimicrobial photodynamic and antibiotic treatments. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v. 119, n. 36, p. 8-pg., 2022-09-06. (13/07276-1, 18/18188-0, 21/09952-0)

DA SILVA, ANA PAULA; ULIANA, MARCIANA PIERINA; GONTIJO GUIMARAES, FRANCISCO EDUARDO; DE OLIVEIRA, KLEBER THIAGO; BLANCO, KATE CRISTINA; BAGNATO, VANDERLEI SALVADOR; INADA, NATALIA MAYUMI. Investigation on the in vitro anti-Trichophyton activity of photosensitizers. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, v. 21, n. 7, p. 8-pg., 2022-03-24. (13/07276-1, 11/19720-8, 19/13569-8, 21/09952-0)

BLANCO, KATE CRISTINA; SILVA, ANA PAULA DA; PANHOCA, VITOR HUGO; MORIYAMA, LILIAN TAN; BAGNATO, VANDERLEI SALVADOR. Photodynamic therapy of adenoid hypertrophy in acute rhinosinusitis. Photodiagnosis and Photodynamic Therapy, v. 39, p. 3-pg., 2022-05-23. (19/12694-3, 13/07276-1, 14/50857-8, 21/09952-0)

ALVES, FERNANDA; STRINGASCI, MIRIAN D.; REQUENA, MICHELLE B.; BLANCO, KATE C.; DIAS, LUCAS D.; CORREA, THAILA Q.; BAGNATO, VANDERLEI S.. Randomized and Controlled Clinical Studies on Antibacterial Photodynamic Therapy: An Overview. PHOTONICS, v. 9, n. 5, p. 36-pg., 2022-05-01. (21/09952-0, 19/12694-3, 13/07276-1, 19/13569-8, 21/01324-0)