A novel antimicrobial drug-delivery coating for percutaneous implant devices

• Grant number: 21/12175-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2021
• End date: August 31, 2022
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Érica Dorigatti de Avila
• Grantee: Beatriz Severino Verza
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated research grant: 18/20719-3 - A novel antimicrobial drug-delivery coating for percutaneous implant devices, AP.JP

Abstract

Antimicrobial coatings on percutaneous devices (PDs), such as dental implants and exposed pins, are considered an effective strategy to avoid infection progression that might result in PD loss. Our conducted preliminary research validated the tetracycline incorporation method into the layer-by-layer system, assembled by alternating polyelectrolyte layers of poly(acrylic acid) (PAA) andpoly(l-lysine) (PLL), through the synthesis of TC with anionic beta-cyclodextrin (²-CD). The succeeded [PAA/PLL]10/TC/anionic ²-CD biomaterial coating showed to be readily tunable to different physiological scenarios, with a controlled release of the drug up to 30 days. Antibacterial effect of [PAA/PLL]10/TC/anionic ²-CD was confirmed against Staphylococcus aureus ATCC 25923. The exciting outcomes encouraged us to proceed with the studies and translate the power of antimicrobial multilayer systems into meaningful biomedical applications. Taking into consideration the importance of tissue quality surrounding PDs and the need to protect the drug into the multilayers, in this project, we will focus on the development of acidic pH sensible film with methacrylic acid (MAA)-based vascular regenerative to cover [PAA/PLL]10/TC/anionic ²-CD and to promote vascularization and subsequent wound healing. Herein, further, in vitro microbiology experiments will be performed to uncover the broad-spectrum of TC/anionic ²-CD against different bacteria involved in implant infection. (AU)