Evaluation of bioglass F18 in skin wounds healing surgically induced in rats

• Grant number: 21/09210-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2022
• End date: December 31, 2022
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Ivan Felismino Charas dos Santos
• Grantee: Letícia Albuquerque Fortes Pereira
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Bioactive glass is a synthetic material based on silica with mechanical properties that form tissue chemical bonds, which have osteoinductive; anti-inflammatory, bactericidal, and healing properties. The F18 bioglass was considered easy to handle and it stimulates the expression of growth factors in vitro, but there are no studies in vivo on the use of the F18 bioglass in uncontaminated skin wounds. Thus, the aim of the study is to evaluate, through a randomized and covered methodology, the effects of F18 bioactive glass in the healing of non-contaminated skin wounds surgically induced in healthy adult Wistar rats. For this, 112 rats were randomly divided into four groups of 28 animals, according to the treatment of 3 cm diameters wounds: Group 1 (G1) - saline solution (0.9%), Group 2 (G2) - allantoin-based healing ointment and zinc oxide (Alantol®); Group 3 (G3) - collagen gel; Group 4 (G4) - collagen gel associated with F18 bioglass. All animals will undergo treatment soon after the wound is made for 21 days. Wounds in Groups G1 and G2 will be treated every 24 hours, and those in Groups G3 and G4 every 72 hours. The variables to be evaluated will include clinical/macroscopic and morphometric parameters, histological and immunohistochemical aspects; and inflammatory activity associated with pro and anti-inflammatory cytokines. The measurement of body mass will be determined 10 minutes before wound induction (M0), three days (M1), seven days (M2), 14 days (M3), and 21 days (M4) after wound induction. Macroscopic evaluation will be performed during wound induction after wound induction and at moments M1, M2, M3, and M4; and body temperature at moments M0 and every 48 hours for 21 days. The values of the area, contraction, and depth of the wounds, as well as the histological, immunohistochemical, and inflammatory cytokine activity, will be determined at moments M1, M2, M3, and M4. Data will be treated for statistical analysis appropriate for each case. (AU)