Development and evaluation of the biological mechanisms of a nanoemulsion loaded with hesperitin on osteoclastogenisis

• Grant number: 21/15178-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2022
• End date: June 30, 2023
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Patricia Milagros Maquera Huacho
• Grantee: Letícia Pereira Lima Durão
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Controlled drug release systems offer countless advantages. Thus, the development of new carrier systems for bioactive drugs mainly of natural origin has become attractive due to their favorable effects on human health. Currently, interest in the use of nanoparticles is growing more and more as a drug delivery system. Among the different nanoparticles, biologically compatible nanoemulsions (NE) offer an optimal administration of drug delivery, controlling its release and directing it to its site of action. Hesperitin (HT) is a flavonoid found mainly in citrus fruits and has excellent pharmacological and biological properties. Studies in the literature and developed by our research group have demonstrated its antioxidant, anti-inflammatory and excellent osteogenicpotential. Due to the biological properties of HT, the aim of this study is to develop ananoemulsion loaded with hesperitin, aiming at a biocompatible, bioactive material with the purpose of enhancing the effect of HT through a controlled drug release system. In the first study, a nanoemulsion loaded with hesperidin (NE-HT) will be developed and characterized. After, in the second study, the effect of non-cytotoxic concentrations of NE-HT on the differentiation and metabolism of osteoclasts in vitro will be evaluated, analyzing the differentiation of osteoclasts by fluorescence microscopy by visualizing an actin ring containing three or more nuclei. Also this study, it will be evaluated the expression of osteoclastogenesis marker genes and protein expression by Western blotting as well as the cell viability of osteoclasts. The numerical data, obtained by the application of laboratory protocols, will be submitted to specific statistical analysis using the GraphPad Prism software, and all tests in this study will be applied with a significance level of 5%. (AU)