HPV variants and polymorphisms analysis existing in European and Latin American women

• Grant number: 22/00712-0
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• Start date: January 30, 2023
• End date: November 29, 2023
• Field of knowledge: Health Sciences - Collective Health - Preventive Medicine
• Principal Investigator: Adhemar Longatto Filho
• Grantee: Luani Rezende Godoy
• Supervisor: Eduardo Luis Fabiano Franco
• Host Institution: Hospital do Câncer de Barretos. Fundação Pio XII (FP). Barretos , SP, Brazil
• Institution abroad: McGill University, Canada
• Associated to the scholarship: 20/00445-6 - Early detection of Cervical Cancer in under- or never-screened women, BP.DD

Abstract

Cervical carcinoma is the third most common type of carcinoma in Brazil. The etiological factor of CC and precursor lesions is persistent infection by the human papillomavirus (HPV). There are several ways to prevent cervical carcinoma. Primary prevention occurs with vaccination. Secondary prevention consists of screening precursor lesions, for which a cytopathological examination of the cervix is performed. However, in some developed countries, the molecular HPV test (DNA-HPV detection) has been used as the primary screening strategy. HPV is classified according to high oncogenic risk (HR-HPV). Among the high-risk types, types 16 and 18 stand out. Inside the types are different variants that have a relation with geographical distribution, pathogenicity, transcript HPV regulation, and immunological host response. Molecular variants intratypic HPV have different oncogenic potential despite the phylogenetic relationship. This study proposes the use of new generation sequencing (NGS) for HPV lineage and variants analysis. HPV genotyping and evaluation of the genetic variability of HR-HPV genotypes has been performed by the Ion Torrent S5 platform in a total number of 1000 samples divided among the four countries participating in the study (Belgium, Brazil, Portugal, and Ecuador. The genotyping was performed with a pipeline developed in the Galaxy platform. For lineage analysis some phylogenetic trees for each HPV type will be. For polymorphisms analysis the variants call will be done. The lineages and polymorphisms found will be analyzed according to clinical data and developing cervical carcinoma relative risk. With this study, we intend to improve the knowledge about the variants and lineages to other HPV types in addition to HPV 16 and 18 present in the study population and analyses if there is a relation between variants and lineages with developing cervical carcinoma relative risk. (AU)