Analysis of cancer cachexia associated with the treatment of drug-carrying nanoparticles of docetaxel and methotrexate in sprague-dawley rats submitted to aerobic physical training

• Grant number: 21/14514-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: July 01, 2022
• End date: September 30, 2024
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Giovana Rampazzo Teixeira
• Grantee: Rafael Ribeiro Correia
• Host Institution: Faculdade de Ciências e Tecnologia (FCT). Universidade Estadual Paulista (UNESP). Campus de Presidente Prudente. Presidente Prudente , SP, Brazil
• Associated scholarship(s): 22/13577-3 - The use of proteomics and RNA-seq techniques for analysis of cancer cachexia associated with the treatment of nanoparticles and aerobic physical exercise, BE.EP.MS

Abstract

Evidence suggests that lipid nanoemulsion for cancer treatment is an effective treatment alternative in the delivery of chemotherapeutic agents to tumor cells. The LDE molecule has great versatility to carry drugs with high dosage and present minimal toxicity, being a new perspective for the fight against some types of neoplasms. The reduction of chemotherapy toxicity led us to test the potential of nanoparticle application in methotrexate therapy in the treatment of cancer cachexia (CC). To increase the specificity of the nanoparticle and the corresponding drug, we will associate aerobic physical training, verifying the impact on cancer cachexia against therapies. Thus, the aim of this study will be to verify the effects of chemically induced cancer cachexia and to evaluate the effects of LDE-methotrexate treatment associated with aerobic physical training on the skeletal muscle of Sprague-Dawley rats. Cachexia will be determined in male Sprague-Dawley rats, which will receive a single dose of 65mg/kg of 7,12-dimethylbenz[a]anthracene (DMBA). After tumor induction, the animals will receive LDE+methotrexate treatments associated with the aerobic physical training protocol. At the end, the animals will be euthanized and the skeletal striated muscle will be collected, weighed and submitted to tissue analysis to evaluate the treatment response in the different groups.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CORREIA, RAFAEL RIBEIRO; CRUZ VERAS, ALLICE SANTOS; TEBAR, WILLIAM RODRIGUES; RUFINO, JESSICA COSTA; GARCIA BATISTA, VICTOR ROGERIO; TEIXEIRA, GIOVANA RAMPAZZO. Strength training for arterial hypertension treatment: a systematic review and meta-analysis of randomized clinical trials. SCIENTIFIC REPORTS, v. 13, n. 1, p. 15-pg., 2023-01-05. (20/15324-0, 21/14514-2, 19/11924-5)

RUBIRA, RAFAEL J. G.; BATISTA, VICTOR R. G.; CORREIA, RAFAEL R.; PAZIN, WALLANCE M.; MAXIMINO, MATEUS D.; RUIZ, GILIA C. M.; TEIXEIRA, GIOVANA R.; JOB, ALDO E.. Biological responses to imazapic and methyl parathion pesticides in bioinspired lipid membranes and Tilapia fish. JOURNAL OF HAZARDOUS MATERIALS, v. 458, p. 12-pg., 2023-06-28. (20/05423-0, 20/15324-0, 20/16310-2, 18/22214-6, 20/15185-0, 21/14514-2)