Advanced search
Start date

Effects of tributyrin supplementation on the gut microbiota of patients with Cancer cachexia

Grant number: 21/14015-6
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): August 01, 2022
Effective date (End): March 31, 2026
Field of knowledge:Biological Sciences - Biochemistry - Metabolism and Bioenergetics
Principal Investigator:Marilia Cerqueira Leite Seelaender
Grantee:Flaydson Clayton Silva Pinto
Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Cachexia is a metabolic disorder characterized by rapid weight loss (muscle and adipose tissue), which affects up to 80% of patients with advanced Cancer, and is related to functional decline and survival. Pro-inflammatory cytokines, such as TNF-± and IL-6, directly contribute to the progression of this condition. The regulation of inflammation, through activation of Toll-like receptors 4 (TLR4) by bacterial lipopolysaccharide (LPS), as well as changes in the microbiota and its metabolism in cachexia, as observed in preliminary experiments of the group, may be all relevant factors in the development and/or progression of the wasting disease. We hypothesize that supplementation of butyrate (in the form of tributyrin), a metabolite that regulates the biology of intestinal microorganisms, may mitigate the symptoms of cachexia in Gastrointestinal Cancer. Therefore, this study aims to characterize the fecal microbiota, (shotgun sequencing), in patients with stable weight or cachectic Gastrointestinal Cancer, after supplementation or not with tributyrin. In addition, plasma biochemical and inflammatory parameters, tumor and healthy stomach or intestine tissue histology, as well as cytokine production in these organs will be investigated. Additionally, an immunofluorescence study will be performed to quantify the nuclear protein Ki-67 in the tumor, and also the expression of CD31, CD45, CD14, CD56, CD3, CD11b, to investigate the characteristics of the tumor microenvironment. Finally, we will analyze the gene expression of interleukins IL-1², -6, -8, -10 and TNF-± in the tumor. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items

Please report errors in scientific publications list by writing to: