Langmuir-Blodgett films of the enzyme laccase for the detection of phenolic derivatives

• Grant number: 22/04241-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2022
• End date: April 26, 2027
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Luciano Caseli
• Grantee: André Campos Machado
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
• Associated research grant: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis, AP.TEM
• Associated scholarship(s): 23/00329-4 - Development of biosensors for phenol compounds utilizing Langmuir and Langmuir-Blodgett films containing laccase, BE.EP.DR

Abstract

In this work we intend to immobilize the laccase enzyme in Langmuir and Langmuir-Blodgett (LB) films of carbon nanotubes functionalized with octadecylamine, as well as octadecylamine associated with conductive polymers such as poly(3-hexylthiophene). It is also intended to immobilize these systems using electrografting (electrografting) associated with the Langmuir-Blodgett technique. We will investigate the physicochemical properties of the prepared films as well as their possible technological application as biosensors and compare the performance of the films formed by LB with the molecular scale properties obtained through thermodynamic and spectroscope data of their respective monolayers at the air-water interface. Characterizations of Langmuir monolayers include surface pressure isotherms, surface potential isotherms, Brewster angle microscopy, vibrational spectroscopy and rheological measurements; and LB films include UV-vis and infrared spectroscopy, atomic force microscopy and quartz crystal microbalance nanogravimetry, as well as feasibility of LB films produced for phenolic derivative sensors through estimation of enzyme activity by colorimetric methods, and voltammetry measurements. The enzyme will also be investigated at the air-water interface by means of monolayers using the salting-out effect. The results will be evaluated against the preservation of catalytic activity, stability of the formed films, detection and quantification limits and their implications in nanobiotechnology. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MACHADO, A. C.; SALVO-COMINO, C.; RODRIGUEZ-MENDEZ, M. L.; CASELI, L.. Enhancing biosensor capabilities through laccase immobilization in nanostructured Langmuir-Blodgett films for phenol detection. RESULTS IN SURFACES AND INTERFACES, v. 18, p. 9-pg., 2024-11-27. (22/03736-7, 18/22214-6, 22/04241-1, 23/00329-4)

DE SOUZA, MATHEUS LIMA; MACHADO, ANDRE CAMPOS; BARBOSA, HENRIQUE; LAGO, JOAO HENRIQUE GHILARDI; CASELI, LUCIANO. Interaction of sakuranetin with unsaturated lipids forming Langmuir monolayers at the air-water interface: A biomembrane model. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 234, p. 7-pg., 2024-01-13. (22/04241-1, 22/09202-4, 22/03736-7)