3D printing: Characterization and in vitro studies of skin dressings manufactured from collagen from fish skin and marine sponges

• Grant number: 22/04991-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: September 01, 2022
• End date: December 31, 2024
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Ana Claudia Muniz Renno
• Grantee: Gustavo Oliva Amaral
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil
• Associated research grant: 19/10228-5 - Interdisciplinar net for the development of biotechnological products from marine origin for use in human health: pathological complication of the osteoarticular system, AP.TEM
• Associated scholarship(s): 23/07615-2 - Enhancing mechanical properties of marine collagens for bone tissue regeneration: physicochemical and mechanical characterization, BE.EP.MS

Abstract

The skin is the largest organ in the body and is constantly vulnerable to trauma and injuries as a result of contact with the external environment; however, it plays an important role in protecting the human body from these attacks. Skin wounds are medical issues that have a negative impact on people's quality of life. Thus, research into therapeutic methods of treating skin wounds is required in order for the patient to recover from the injury as efficiently as possible while incurring the least amount of treatment costs. Therefore, it is predicted that 3D-printed marine sponge's collagen and fish skin collagen dressings will exhibit effective physicochemical and biological properties for cutaneous repair. With this in mind, the aims of this work are: (I) Manufacture of marine sponge and fish skin collagen dressings using 3D printing techniques; (II) To characterize the dressings' morphological and physicochemical properties; (III) Evaluate the dressings' biocompatibility and cell viability. Colections of marine sponges and fish will be carried out in accordance with their respective protocols for later collagen extraction. The ink will be produced at a concentration of 4% sodium alginate in a mass ratio with marine collagen of 1:9. Then will be print a dressing with dimensions of 20 mm in diameter and 2 mm high. Following, the dressings will be freeze-dried and sterilized with UV to send them to the characterization tests (pH, degradation, hydration profile, FTIR, SEM and porosity) and the in vitro tests (Cell Adhesion and MTT assay). Following that, the data will be presented for mean and standard deviation, graphs and tables, the Shapiro Wilk normality test, and the ANOVA-Two-way analysis of variance. (AU)