Pesticides are important components in the control of pests that affect plants, animals and humans. The intensive use of these chemicals, however, has implications for the ecosystem and human health. Due to its wide use and repeated applications, residues of fungicides, including tebuconazole (TEB), are part of the wide spectrum of pesticides found in water and food consumed by Brazilians. TEB is a triazole fungicide, recognized for inhibiting ergosterol biosynthesis and its high physicochemical stability ensures its persistence in the environment, where it can act on non-target species, including humans. It is described that exposure to TEB can result in toxicity to various organs, such as the liver, kidneys, brain and heart. Furthermore, maternal exposure to TEB during pregnancy results in problems with fetal development. Knowing that women of childbearing age are at increased risk of dietary exposure to TEB and that maternal exposure to environmental toxins is associated with a greater predisposition to develop heart problems in their children after birth and adulthood, we aim to investigate the effects cardiotoxic effects of tebuconazole in offspring mice after maternal exposure to the fungicide during pregnancy and lactation, specifically, assess the effects of TEB on: (1) markers of cardiac injury and lipid profile, through biochemical analyzes of sérum samples; (2) cardiac tissue remodeling, through morphometric and histopathological analysis of the heart; (3) oxidative stress and (4) cardiac electrical activity, through electrocardiography techniques (in vivo and ex vivo) and in vitro through the patch-clamp technique applied to isolated cardiomyocytes. At the end, we will be able to determinate if maternal exposure to TEB is able to induce heart remodeling to the offspring, which may impact the use of TEB in agriculture.
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