Nanoionomics applied to soybean cultivation

• Grant number: 22/13166-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): November 01, 2022
• Effective date (End): October 31, 2024
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Marco Aurelio Zezzi Arruda
• Grantee: João Gabriel Veneziani Kamezawa
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 18/25207-0 - Nanometallomics applied to soybean cultivation for studying the effects of metal-related nanoparticles, AP.TEM
• Associated scholarship(s): 23/05547-0 - Developing a characterization method for iron-oxide nanoparticles through the hyphenated technique using LC-ICP-MS, BE.EP.IC

Abstract

The production and exportation of soybean are essential for the Brazilian economy. Therefore, advancing biotechnology techniques to improve this grain's quality and productivity is of interest to the country. Various techniques already assist and/or have great potential to improve soybean production. One of them is somatic embryogenesis, which may be used as a model to study embryogenic development or obtain many embryo cells. In this technique, the plant cells suffer a dedifferentiation process forming plants callus cultured in vitro, forming a significant number of embryos. To improve this technique is possible to employ nanoparticles. Due to the many properties of nanoparticles, they may interfere with the callus homeostasis, changing its development and propagation. Thus, the present work aims to investigate the disturbance of silver nanoparticles (40, 60, and 80 nm), added to the culture medium, at the ionomic callus of transgenic soybeans. These callus samples will be prepared by microwave-assisted decomposition and high-frequency ultrasonic (CupHorn) extraction to optimize the sample preparation process. The elementar determination will be effected through inductively coupled plasma mass spectrometry (ICP-MS), and the control samples will be compared with the samples treated with nanoparticles.