"Actuation of faveleira propolis in second intention skin healing. A study focusing on dermal fibroblasts"

• Grant number: 22/09801-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): February 01, 2023
• Effective date (End): July 31, 2023
• Field of knowledge: Biological Sciences - Morphology - Histology
• Principal Investigator: Claudia Helena Pellizzon
• Grantee: Andréia Cristina Miranda Alves
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated research grant: 17/04138-8 - Attainment of chemical, analytical, biological, pharmacological and technological studies to fill the gaps on the development of Brazilian propolis sector, AP.TEM

Abstract

Skin wound is defined as a situation whose skin integrity is impaired, either by accidents, trauma, illness or surgery. Skin lesions can be quickly healed or require a long time to complete this process. Tissue repair is among the most complex processes in the human body, and for that it requires the synchronization and activities of several different cell types in stages that may overlap. Among the cells involved, we highlight the fibroblast, whose main function is to maintain the integrity of the connective tissue, where it deposits and remodels the extracellular matrix. Fibroblasts exhibit great plasticity and are responsive to chemical signaling from the epidermis and other cells of the dermis, being able to differentiate into myofibroblasts that exert contractile functions so that in the healing process the wound is closed, and it is believed that because fibroblasts synthesize and organize the extracellular matrix, are responsible for the formation of scars that can be pathological (hypertrophic and keloids).Despite advances regarding the development of resources and technology for tissue repair, and there is no gold standard for treatment, there is a need to investigate new therapies for this area, and phytotherapy can be explored for this purpose, since Brazil is rich in biodiversity, and policies related to phytotherapy already exist. Our objective is to investigate the healing effect of propolis from the plant Cnidoscolus Quercifolius, known as faveleira in second intention wounds in rats, through histopathological and immunohistochemical macroscopic and microscopic parameters and to morphometrically evaluate the fibroblasts in the healing process.