Expression of Set1/Trithorax-type H3K4 (histone 3 lysine 4) methyltransferases during skeletal muscle regeneration

• Grant number: 23/00289-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2023
• End date: March 31, 2025
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Elen Haruka Miyabara
• Grantee: Alana Moreira de Santana
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Skeletal muscle tissue has the ability to regenerate after injury. Satellite cells are stem cells specific of the skeletal muscle and play a fundamental role in the regeneration of this organ. Although the morphological aspects of skeletal muscle regeneration are well known, the molecular mechanisms of this process are still not completely understood. Considering that the Set1/Trithorax-type H3K4 methyltransferases Mll1/2 (Mixed-lineage leukemia 1/2), and histone methyltransferase complex are involved in satellite cell divisions and activation of myogenic genes, respectively; and that Mll1 is required for the expression of the transcription factor Pax7 and satellite cell proliferation and self-renewal in mice, we hypothesize that the Set1/Trithorax-type H3K4 methyltransferases play important roles in the skeletal muscle regenerative process. Therefore, this project aims to analyze the expression of the Set1/Trithorax-type H3K4 methyltransferases Setd1a, Setd1b, Mll1, Mll2, Mll3 and Mll4 during skeletal muscle regeneration (please check details in the project).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTOS, AUDREI R.; KOIKE, TATIANA E.; SANTANA, ALANA M.; MIRANDA, NATALYA C.; DELL AQUILA, RODRIGO A.; SILVA, THIAGO C.; AOKI, MARCELO S.; MIYABARA, ELEN H.. Glutamine supplementation accelerates functional recovery of EDL muscles after injury by modulating the expression of S100 calcium-binding proteins. Histochemistry and Cell Biology, v. 160, n. 2, p. 12-pg., 2023-05-14. (20/15351-7, 17/09069-4, 23/00289-2, 18/24946-4)