The influence of subcutaneous doxycycline on alveolar bone remodeling in osteopenic rats

• Grant number: 22/15982-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2023
• End date: April 30, 2024
• Field of knowledge: Health Sciences - Dentistry - Orthodontics
• Principal Investigator: Maria Bernadete Sasso Stuani
• Grantee: Antonio Giroto dos Santos
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Introduction: The extraction of dental elements is still a very common fact in the dental clinic, and may be due to trauma, periodontal problems or dental caries. It is known that, when treatment with implants or orthodontic tooth movement is indicated, in patients with established osteoporosis, a longer post-treatment time is necessary, and that there is a correlation between present osteoporosis and mandibular/maxillary trauma resulting from lack of of bone tissue in the region. Objective: This project aims to compare the use of doxycycline in Wistar rats, in subantimicrobial doses (SDD), and sodium alendronate in the treatment of bone remodeling after tooth extraction. Materials and Methods: 72 Wistar rats will be used, divided into 6 groups (n=6), with the groups defined as follows: C - Control; CDOX - Control exposed to doxycycline 30 mg/kg/day; CAL - Control exposed to sodium alendronate 0.7 mg/kg/week; OVX - Ovariectomized Control; OVXDOX - Ovariectomized patient exposed to doxycycline 30 mg/kg/day; OVXAL - Ovariectomized patient exposed to sodium alendronate 0.7 mg/kg/week. The rats, around 80 days old, will undergo the osteopenia induction procedure for 90 days. After this period, the rats will be submitted to treatment with doxycycline at a dose of 30 mg/kg/day or alendronate sodium at a dose of 0.7 mg/kg/week, both for 60 days. The total experimental period will be 150 days, fourteen and seven days before sacrifice, all animals will be submitted to tooth extraction. After 150 days from the beginning of the experiment, euthanasia will be performed. Samples from alveoli undergoing repair will be submitted to histological, histomorphometric and immunohistochemical analysis by TRAP. Statistical analysis: The results of this project will be submitted to the Anova statistical test.