HUMAN BOCAVIRUS MOLECULAR EPIDEMIOLOGY FROM HISTORICAL FECAL SAMPLES IN BRAZIL (1998-2005)

• Grant number: 22/02529-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2023
• End date: November 30, 2023
• Field of knowledge: Health Sciences - Collective Health - Public Health
• Principal Investigator: Adriana Luchs
• Grantee: Ellen Viana de Souza
• Host Institution: Instituto Adolfo Lutz (IAL). São Paulo , SP, Brazil

Abstract

Human Bocaparvovirus (HBoV) was described in 2005, during a study aimed at investigating the presence of etiological agents involved in respiratory infections in hospitalized children in Sweden. There are 4 HBoV genotypes identified so far. HBoV-1 is commonly associated with respiratory infections, while HBoV-2, HBoV-3 and HBoV-4 are often detected in faecal specimens. HBoV has a global distribution and epidemiological studies worldwide confirm its importance as an etiological agent associated with both diarrheal disease and respiratory disease. Co-infections involving HBoV and other enteric viral agents are common. In Brazil, the first detection of HBoV occurred in 2007 in fecal samples from children with acute gastroenteritis. The aim of the present study is to investigate the frequency and genotypic diversity of HBoV in historical fecals stored and collected before 2005, in order to understand the natural history of HBoV in patients with diarrhea in Brazil before the introduction of the rotavirus vaccine and before its description. This will be a retrospective study conducted with feces from the national rotavirus surveillance collected between 1998 and 2005. The samples included in the study will be tested for HBoV by real-time PCR (qPCR) targeting the gene coding for the NS1 and NP1 genes. Genotyping of HBoV positive samples will be performed by conventional PCR targeting VP1/2 genes, followed by sequencing. HBoV positive samples obtained during the study period will be selected for phylogenetic analysis. The present study will increase the epidemiological knowledge of infections caused by HBoV, as well as their genetic diversity. Data from this study will also contribute to a better understanding of the role of HBoV in gastroenteritis in the country before rotavirus vaccination introduction, as well as historical samples collected prior to its description in 2005. (AU)

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

FRANCA, YASMIN; MEDEIROS, ROBERTA SALZONE; VIANA, ELLEN; DE AZEVEDO, LAIS SAMPAIO; GUIDUCCI, RAQUEL; DA COSTA, ANTONIO CHARLYS; LUCHS, ADRIANA. Genetic diversity and evolution of G12P[6] DS-1-like and G12P[9] AU-1-like Rotavirus strains in Brazil. FUNCTIONAL & INTEGRATIVE GENOMICS, v. 24, n. 3, p. 40-pg., 2024-06-01. (20/11182-6, 17/50333-7, 20/02469-0, 20/14786-0, 22/02529-8, 21/09064-8, 15/12944-9)

LUCHS, ADRIANA; ADIWARDANA, NATANAEL SUTIKNO; DA ROCHA, LEONARDO CECILIO; VIANA, ELLEN; GUADAGNUCCI, SIMONE; PARISE, ADRIANA; SILVA, VANESSA CRISTINA MARTINS; DE AZEVEDO, LAIS SAMPAIO; GUIDUCCI, RAQUEL; FRANCA, YASMIN; et al. Concurrent Circulation of Viral Agents in Pediatric Patients Presenting with Respiratory Illness and Diarrheal Symptoms in Metropolitan Region of São Paulo, Brazil, 2021. Viruses-Basel, v. 17, n. 4, p. 16-pg., 2025-03-29. (15/12944-9, 20/14786-0, 22/02529-8, 21/09064-8)