In swine production, respiratory diseases have a high prevalence and generate a big economic impact. Porcine Enzootic Pneumonia (PES), associated with infection by Mycoplasma hyopneumoniae (M. hyopneumoniae), is considered an important predisposing factor for secondary infections that corroborate with greater severity of lesions and the development of pleurisy. Based on this, the present study aims to investigate the main genotypes of M. hyopneumoniae associated with different degrees of pleurisy, using the Multilocus sequence typing (MLST) technique, relating the genotype to the severity of the lesion observed at slaughter. For this, pig lung samples will be collected from at least 5 different farms, these will be divided into 5 groups according to the severity of the pleurisy lesions observed. The samples will be submitted to the qPCR technique for the detection of M. hyopneumoniae, and at least one positive sample from each group will be submitted to the MLST technique. It will also be performed qPCR aiming the detection of Pasteurella multocida and Actinobacillus pleuropneumoniae, to evaluate possible co-infections. The normality of the variables will be verified by the Shapiro-Wilkins test. If there is normality, analysis of variance (ANOVA) will be applied, the simple t test will be used for comparison between groups. If there is no normality, non-parametric tests will be used. The results will allow a better understanding of the pathogenicity characteristics of the strains at the field level, in addition to generating data that will make it possible to monitor the dynamics of this agent's circulation in the herds, allowing the understanding of the epidemiology of M. hyopneumoniae, providing a better assertiveness of control programs and prevention of ESP.
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