Immobilization of graphene oxide and laccase on lipid Langmuir-Blodgett films: possible application for biosensors for phenolic compounds

• Grant number: 23/04976-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2023
• Status: Discontinued
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Luciano Caseli
• Grantee: Felipe Merloto Marinho
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
• Associated research grant: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis, AP.TEM
• Associated scholarship(s): 24/16693-0 - Development of biosensors for phenol compounds utilizing Langmuir-Blodgett films containing graphene oxide and laccase, BE.EP.IC

Abstract

In this work we intend to study the interaction of the laccase enzyme in Langmuir and Langmuir-Blodgett (LB) films of dimyristoylphosphatidic acid/graphene oxide. The lipid will be spread at the air-water interface and the enzyme, as well as graphene oxide dispersions, will be inserted into the supporting aqueous subphase of the lipid monolayer. The adsorption of the enzyme to the monolayer will be studied by tensiometry (surface-area pressure isotherms, stability of previously compressed monolayers, hysteresis tests), surface-area potential isotherms, rheological measurements (oscillating barriers and interfacial shear), vibrational spectroscopy and microscopy at the Brewster angle. The mixed monolayer will be transferred to solid supports by LB methodology, and the enzymatic activity of laccase will be evaluated against phenolic compounds.