Study of the antioxidant and antiglycation potential of the hydroethanolic extract of Cissus verticillata leaves in in vivo model system of obesity and in vitro model system of glycoxidative stress

• Grant number: 23/03464-0
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Effective date (Start): August 01, 2023
• Effective date (End): February 28, 2027
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Amanda Martins Baviera
• Grantee: Felipe Nunes Cardoso
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Diabetes mellitus (DM) is a chronic endocrine disease characterized by insulin resistance in target tissues and pancreatic dysfunction. The DM, especially type 2, is closely associated with an increase in the prevalence of obesity. Most of the diabetic complications are related to the increase in the generation of advanced glycation end products (AGEs) and reactive oxygen species (ROS), which are responsible for promoting structural damages to several biomolecules and activating pro-inflammatory pathways. In order to increase the effectiveness of the DM treatment, combined therapy strategies between classic antidiabetic agents and natural products have been suggested. Cissus verticillata (L.) Nicolson & C.E. Jarvis is a plant species whose preparations obtained from its leaves have already demonstrated antidiabetic properties, both in rodents and humans. Therefore, this study aims to investigate the changes in physiometabolic parameters and biomarkers of glycoxidative stress in mice fed a high-fat diet (in vivo model system of obesity/insulin resistance) treated with the hydroethanolic extract of leaves from Cissus verticillata, alone or in combination with metformin, as well as to evaluate the anti-AGE potential of the extract through an in vitro model system of protein glycation. (AU)