Antibiofilm activity of indocyanine green laser ablation compared to photodynamic therapy with methylene blue and curcuma in root canals contaminated with Candida albicans and Enterococcus faecalis

• Grant number: 23/05137-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2023
• End date: July 31, 2024
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Rogerio de Castilho Jacinto
• Grantee: Nayara Gabriely Dourado
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Antimicrobial photodynamic therapy (aPDT) is used as an adjunct to endodontic treatment to enhance microbial reduction. This in vitro study aims to evaluate the efficiency of laser ablation with indocyanine green compared to aPDT using the photosensitizer methylene blue (MB) and curcumin (CUR) in reducing duo-species biofilm of Candida albicans and Enterococcus faecalis in canals roots. The specimens will consist of 100 bovine teeth, divided into five groups, according to the decontamination protocol: MB+RL (n=20) (Methylene Blue + Red Laser); CUR+BL (n=20) (Curcumin + blue LED); ICG+DL (n=20) 2.5/300/100 (Indocyanine Green + infrared diode laser 2.5 W power, 300 ms interval and 100 ms duration); negative control (n=20) and positive control group (n=20). The collection of root canal content will be performed 21 days after contamination (S1) and immediately after the different treatment protocols (S2). Each canal will be filled with sterile saline solution (1 ml), and three tips of sterile absorbent paper #80 (Dentsply Maillefer, Switzerland) will be inserted into the canals for 1 minute. Each sample will be homogenized and diluted. The dilutions will be cultivated in Sabouraud Dextrose agar plates with chloramphenicol and Enterococcosel agar and incubated for 48 hours at 37ºC. Microbial growth will be determined by counting the number of colony-forming units (CFU/ml). The collected data (CFU/ml) will be statistically analyzed, and a comparison before and after decontamination will be performed using the Two-way ANOVA test and the Student-Newman-Keuls test, (p<0.05).