Extraction, separation and two-dimensional analysis of phenolic compounds from agro-industry residues with antimicrobial potential against Staphylococcus aureus for application in curatives.

• Grant number: 23/03439-5
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: November 01, 2023
• End date: October 31, 2026
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Principal Investigator: Maurício Ariel Rostagno
• Grantee: Thais Carvalho Brito Oliveira
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated research grant: 18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals, AP.JP2

Abstract

This research project aims to evaluate the extraction, separation and bidimensionalanalysis of phenolic compounds from agro-industry residues with antimicrobialpotential against Staphylococcus aureus for application in curatives. Initially, fastanalysis methods will be developed for the identification and quantification by ultrahigh pressure liquid chromatography (UHPLC) of the compounds present in samples ofdifferent agro-industry residues. The fractionation of compounds present in thesesamples will be studied through the on-line coupling of pressurized liquid extraction(PLE) in gradient with solid phase extraction (SPE) (PLEgrad-SPE) with real-timemonitoring. Real-time monitoring of the extraction/separation process will be carriedout through the orthogonal coupling PLEgrad-SPE x UHPLC and the simultaneouselaboration of kinetic extraction curves of several compounds present simultaneously.The compounds present will be identified and two-dimensional profiles of the chemicalcomposition of the fractions will be prepared obtained through orthogonal couplingPLEgrad-SPE x UHPLC. Fractionated extracts of samples will be obtained to carry outthe evaluation of antimicrobial activity against Staphylococcus aureus and selection offractions with greater activity. The best way to apply the selected phenolic compoundsin films for application in curtives will be evaluated, for which: (i) the necessity ofencapsulating the bioactives will be tested; (ii) films containing the bioactives will bedeveloped, testing different options: chitosan, gelatin and/or starch films, and (iii) thefilms will finally be characterized for later application in curatives/dressings.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE LANA, VALERIA SILVA; ESTEVAM, PATRICIA NAYARA; DE CASTRO, THAIS BARCELOS; JOSE, VINICIUS PARZANINI BRILHANTE DE SAO; BRITO-OLIVEIRA, THAIS CARVALHO; SANTOS, PEDRO HENRIQUE; OLIVEIRA, CRISTIANE ALMEIDA SANTOS; CORREA, CRISTIANE BANI; ROSTAGNO, MAURICIO ARIEL; MARTINO, HERCIA STAMPINI DUARTE; et al. Nutritional and technological potential of umbu-caja and soursop co-product flours. Food Research International, v. 200, p. 12-pg., 2024-12-23. (19/13496-0, 23/11025-6, 18/14582-5, 23/03439-5)

PENA, FABIOLA DE LIMA; DE SOUZA, MARIANA CORREA; SANCHES, VITOR LACERDA; VIGANO, JULIANE; MANCINI, MARIANA CAMARGO SILVA; BRITO-OLIVEIRA, THAIS CARVALHO; DOS SANTOS, BARBARA GECIANA TOMAZ; DE QUEIROZ, GUILHERME HENRIQUE AGUIAR; TAMBORLIN, LETICIA; SIMABUCO, FERNANDO MOREIRA; et al. Phenolic profile, bioactivity and cytotoxicity of plant extracts from thyme, ginger, garlic, ground roasted coffee and coffee silverskin. INTERNATIONAL JOURNAL OF FOOD PROPERTIES, v. 28, n. 1, p. 18-pg., 2025-12-31. (23/03439-5, 18/14818-9, 19/13496-0)