ROLE OF ANGIOTENSIN II ON NORADRENALINE-INDUCED STRESS IN HUMAN SQUAMOUS CELL CARCINOMA (SCC-25, SCC-9 e NOK-SI) CELL LINE FOR IL-6 AND TNF-± PRODUCTION.

• Grant number: 23/08457-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2024
• End date: February 28, 2025
• Field of knowledge: Biological Sciences - Pharmacology
• Principal Investigator: Sandra Helena Penha de Oliveira
• Grantee: Beatriz Travalon Fabricio
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Squamous cell carcinoma (SCC) is a malignant neoplasm that corresponds to approximately 95% of cases of oral cancers. In addition to smoking and alcoholism, stress also contributes to the development of cancer. Data in our group demonstrated that the treatment of squamous cell carcinoma (SCC-25) cells stimulated by norepinephrine (NOR) induces the production of higher levels of interleukin-6 (IL-6), which is an inflammatory cytokine related to chronic stress that is important to promote angiogenesis and tumor progression. It is known that inflammation is very important for the tumor environment. One of the components of the renin-angiotensin system, angiotensin II (Ang II), also functions as one of the mediators of the inflammatory process, playing an important role in the process of cancer development since it plays a regulatory role in binding to its AT1R (pro-inflammatory) and AT2R (anti-inflammatory) receptors. Thus, the aim of the present study will be to evaluate the role of Ang II on the stress induced by NOR in squamous cell carcinoma (SCC-25) cell lines for the production and expression of IL-6 and TNF-±. Therefore, the culture of tongue cancer strain cell line (SCC-25) will be stimulated by NOR and Ang II and we will evaluate the production and expression of IL-6 and TNF-± by ELISA and real-time RT-PCR respectively. Certainly, this study may collaborate with new therapies and new visions for the treatment of people with squamous cell carcinoma