Effect of resistance exercise on insulin resistance in skeletal muscle in non-obese type 2 diabetic animal model

• Grant number: 23/03499-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2024
• End date: May 31, 2027
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Sandro Massao Hirabara
• Grantee: Luiz Eduardo Ferraz Rodrigues
• Host Institution: Centro de Ciências Biológicas e da Saúde. Universidade Cruzeiro do Sul (UNICSUL). São Paulo , SP, Brazil
• Associated research grant: 18/09868-7 - Cellular and molecular mechanisms of insulin resistance and inflammation in obese Wistar rats and lean Goto-Kakizaki rats: causes and associations with diet and physical exercise, AP.TEM

Abstract

Overweight and obesity are considered risk factors for the development of type 2 diabetes mellitus (DM2), with numerous metabolic diseases. However, it is also observed that a significant portion of the population develops DM2 without excess weight, that is, in thin individuals, especially in eastern countries, where the rate of type 2 diabetics without obesity reaches 60%. In Brazil, the estimate of patients with DM2 without obesity can reach 20%. Loss of muscle mass is common in DM2, being associated with several physical and metabolic disorders in these patients. Thus, strategies aimed at mitigating or reducing muscle loss are important in DM2. Physical exercise, such as strength training, can be used both for the prevention and treatment of this pathology; however, there are still limited works that address trained protocols and that guide training for the best benefits involved in this population. Thus, this project will better understand the pathophysiological mechanisms involved in the development of DM2 without obesity, as well as evaluate potential strategies, such as resistance training, quantifying training volume and performance. For this, Goto-Kakizaki rats (animal model of T2DM without obesity) and the ladder apparatus (bodybuilding training model), established by Hornberg & Farrar (2004), will be used in this work. The training will consist of a period of 12 weeks, following the protocols for comparison of volume and load, applying techniques that will be able to provide the cellular and molecular relationships in the exercise intervention for the DM2 model without obesity. At the end of the experimental protocol, the effects of training on several parameters, including muscle mass, insulin resistance and inflammation, will be evaluated. Thus, we intend to determine the cellular and molecular controls involved in the visual effects of resistance training in DM2 without obesity (Goto-Kakizaki rats), seeking preventive and/or therapeutic strategies for patients affected by this disease. (AU)