EEVALUATION OF THE OXIDATIVE STRESS IN SKELETAL MUSCLE TISSUE OF ADULT RATS, OFFSPRING OF RATS WITH TRUE ENDO-PERIODONTAL LESION

• Grant number: 24/22879-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2025
• End date: January 31, 2026
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Doris Hissako Matsushita
• Grantee: Mariana Campanha Hilário Pereira
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Fetal programming suggests that adverse stimuli when applied during early fetal development can alter the offspring's metabolism, increasing the risk of disease in their adult life. The true endo-periodontal lesion (EPL) is an infectious process that extends to the pulp and periodontal tissue simultaneously, each with its origin. It was found that this pathology is related to more expressive changes in IR, insulin signal (SI) and inflammatory pathways compared to AP and PED evaluated separately. Maternal EPL promoted the activation of inflammatory pathways in the gastrocnemius muscle (GM) of the adult offspring, a fact confirmed by the increased expression of tumor necrosis factor-alpha (TNF-¿) and the phosphorylation status of kappa B inhibitor kinase , which stimulates the activation of the nuclear factor kappa B (NF-kB) that act by increasing the transcription of pro-inflammatory mediators, such as TNF-¿, in addition to being related to the repression of the Slc2a4 gene, responsible for encoding the protein transporter type 4 glucose (GLUT4). However, no difference was found in the phosphorylation status of the c-Jun aminoterminal kinase protein, which promotes the phosphorylation of the insulin receptor substrate (IRS1) in serine, attenuating the IS. It is known that oxidative stress is a mechanism implicated as a contributing factor in both the and progression of diabetes. The aims of the present study will be to verify in adult rats, offspring of rats with EPL: 1) Insulin sensitivity; 2) oxidative stress in GM. For that, the 28 Wistar rats (2 months old) will be distributed in 4 groups: 1) control rats; 2) rats with an induced AP in the maxillary right first molar; 3) rats with an induced PED in the upper right second molar; 4) rats with EPL, in which PED will be induced in the maxillary right second molar, and AP in the maxillary right first molar. After 30 days of induction of oral inflammation, rats from all groups will be placed for mating with healthy rats. When the male offspring of these rats are 75 days old, the following experiments will be performed: 1) glycemia and insulinemia, followed by the calculation of the Homeostasis Assessment Model of Insulin Resistance (HOMA-IR); 2) oxidative stress in GM. Statistical analysis will be performed by analysis of variance, followed by Tukey's test (p<0.05).