Biological and physicochemical analysis of endodontic bioceramic sealers and repair cements in rat subcutaneous tissue

• Grant number: 24/15219-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2025
• End date: February 28, 2026
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Mario Tanomaru Filho
• Grantee: Laís Ventura Barroti
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Introduction: Calcium silicate cements have been developed as restorative materials and endodontic cements, with emphasis on biocompatibility and bioactivity. Objective: This project aims to evaluate the biological (biocompatibility and bioactive potential) and physicochemical (porosity and dentin-material interface) properties of endodontic bioceramic filling and repair cements. Materials and methods: The project will be divided into two sub-projects. Sub-project 1 - evaluation of BioRoot Flow (BRF) bioceramic endodontic cements compared to BioRoot RCS (BRRCS) and Bio-C Sealer (BCS) cements. Sub-project 2 - evaluation of NeoMTA2 (NMTA2) bioceramic repair cements compared to Bio-C Repair (BCR) and Biodentine (BD) cements. The tissue reaction caused by the different materials in the subcutaneous tissue of rats (n= 6/group/time period) will be assessed by implanting dentin tubes filled with the materials. After 7, 15, 30 and 60 days, the tubes containing adjacent connective tissue will be removed and processed to assess biocompatibility and bioactive potential. The dentin tubes will be removed from the adjacent tissues and prepared for micro-CT analysis of porosity and the dentin-material interface. Sections of subcutaneous tissue will be stained with hematoxylin and eosin (H&E) to analyze the numerical density of inflammatory cells and the thickness of the capsules. Other sections adhered to silanized slides will be used for immunohistochemical reactions to detect interleukin-6 (IL-6), a mediator in the inflammatory response, and osteocalcin (OCN), a protein secreted by osteoblasts in the mineralization process. The Von Kossa method will be used to identify possible birefringent structures in the capsule adjacent to the tubes. Analysis of results: The results will be analyzed and compared using specific statistical tests for each case and with a significance level of 5%.