Evaluation of the possible beneficial effect of probiotic nanocomposite on oral damage associated with inflammatory bowel disease induced in mice.

• Grant number: 25/08261-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2025
• End date: September 30, 2026
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Gisele Alborghetti Nai
• Grantee: Silmara Gomes Pinheiro
• Host Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil

Abstract

Inflammatory bowel disease (IBD) is a chronic, relapsing and remitting inflammatory disease of the gastrointestinal tract with multifactorial etiology. In IBD, intestinal immunomodulatory mechanisms are unbalanced, leading to persistent inflammation. Oral manifestations of IBD can occur due to several factors. Food bioactive compounds have great health benefits due to their anti-inflammatory and antioxidant effects. OBJECTIVE: To evaluate the effects of the use of nanoprobiotics and nanoprebiotics on oral damage associated with indomethacin-induced IBD in mice. MATERIAL AND METHODS: This study will use stored material from the project "Evaluation of oral damage associated with intestinal damage caused by indomethacin and possible beneficial effect of curcumin nanoemulsion and nanoprobiotics - an experimental study in mice", approved by CEUA, process number 7988, under the responsibility of Gisele Alborghetti Nai. Fifty-four male Mus musculus (C57BL/6) mice, 6-8 weeks of age (weight between 18-22g), were allocated into 6 groups to perform the experiments. For IBD induction, the animals received indomethacin (10mg/kg) orally. The treatments used were: isolate with potential probiotic Pediococcus acidilactici CE51 108UFC/ml; chitosan nanocomposites associated with the isolate with potential probiotic Pediococcus acidilactici CE51 108UFC/ml; chitosan nanocomposite; and sulfasalazine (10mg/kg) administered orally. After euthanasia, a fragment of the buccal mucosa from each side and the tongue were collected for histopathological analysis. EXPECTED RESULTS: It is expected to observe oral damage associated with intestinal damage and improvement of this with the use of probiotics.