Photodynamic therapy with chlorin-e6 (Ce6) gel containing enamel matrix derivatives (EMD) in the treatment of peri-implantitis

• Grant number: 25/01084-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: January 01, 2026
• End date: December 31, 2028
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Denise Madalena Palomari Spolidorio
• Grantee: Gabriel Garcia de Carvalho
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Mechanical debridement is indicated for the treatment of peri-implant disease. However, this approach may present insuficient outcomes when used alone in certain clinical cases. Therefore, alternative therapies have been investigated to overcome its limitations related to limited access to the implant surface and the persistent presence of bacteria. Among these therapies, photodynamic therapy (PDT) and the use of enamel matrix derivatives (EMD) stand out. The Study 1 aim to evaluate, in vitro, the potential of resolution of photodynamic therapy with chlorin-e6 (Ce6) in gel containing enamel matrix derivatives in peri-implantitis, as an antimicrobial and healing agent. Initially, Objective 1, the elimination capacity of PDT with Ce6 in gel containing EMD on the planktonic bacteria related to peri-implantitis will be evaluated. Next, Objective 2, the antimicrobial action of PDT with Ce6 in gel containing EMD against single and multispecies biofilm of bacteria associated with peri-implantitis will be tested. Finally, Objective 3, the effect of PDT with Ce6 in gel containing EMD on osteogenesis under normal and inflammatory conditions will be evaluated, considering cell proliferation and migration, quantification of mineralization nodules and alkaline phosphatase activity. The Study 2 aim to evaluate the clinical, microbiological and immunological profile of patients undergoing peri-implant treatment with PDT sessions with Ce6 in gel containing EMD. In study 2, a randomized controlled clinical trial will be developed to test a new treatment protocol for peri-implantitis, testing PDT with Ce6 in gel containing EMD as an adjuvant to basic surgical treatment, considering clinical, radiographic, microbiological and immunological parameters. All data will be subjected to statistical analysis with a significance level of 5% (p<0.05). (AU)