Identification of trans-activating factors that regulate BhC4-1 gene expression in the ring gland

• Grant number: 06/06081-9
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: April 01, 2008
• End date: March 31, 2011
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Nadia Monesi
• Grantee: Juliana Aparecida Candido da Silva
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The Bradysia hygida (Diptera, Sciaridae) DNA puff C4 BhC4-1 gene is amplified and expressed in a developmentally regulated manner in the salivary gland at the time DNA puff C4 is formed. Since it is not possible to perform functional assays in Sciaridae, the BhC4-1 regulation is studied in Drosophila, a system in which the pattern of BhC4-1 expression is largely conserved. In transgenesis the onset of BhC4-1 expression is verified in prepupal salivary glands and in the ring gland of late embryos. The CIs-regulatory elements which control gene expression in the salivary gland are separable from those which regulate gene expression in the ring gland. Very little is known about ring gland specific transcription factors. In this sense, this project aims to identify trans-activating factors which regulate BhC4-1 gene expression in the ring gland employing the yeast one-hybrid system, a tool which has been widely employed to identify transcription factors. The obtained results will contribute for the understanding of the regulatory mechanisms which control developmentally regulated and tissue-specific gene expression. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CANDIDO-SILVA, J. A.; MONESI, N.. Bradysia hygida (Diptera, Sciaridae) presents two eukaryotic Elongation Factor 1A gene homologues: partial characterization of the eukaryotic Elongation Factor 1A-F1 gene. Brazilian Journal of Medical and Biological Research, v. 43, n. 5, p. 437-444, MAY 2010. (06/06081-9)