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Influence of inflammatory response in alveolar bone repair in homeostatic and infectious conditions: morphometric characterization in mice genetically selected for maximal or minimal inflammatory reaction

Grant number: 10/10379-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2010
Effective date (End): August 31, 2012
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Gustavo Pompermaier Garlet
Grantee:Raíssa Gonçalves Carneiro Spera de Andrade
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

Bone metabolism is influenced by endocrine, genetic and growth factors, RANK/RANKL/OPG system, besides a variety of regulatory molecules such as cytokines. However, little is known about the mechanisms involved in the interaction between skeletal and immune system in the bone repair process, especially in relation to alveolar bone repair. The alveolar repair process after tooth extraction can be used as an experimental model to clarify the action mechanisms of different cytokines on bone metabolism both in normal (homeostatic, when the repair occurs without disruption) as in infectious conditions, as in experimental dry socket, which results in intense inflammatory response and in delayed alveolar repair. Therefore, the objective of this project is to elucidate the influence of inflammatory response in alveolar repair process after tooth extraction in homeostatic and infectious (experimental dry socket) conditions in different time points (0, 7, 14 and 21 days) in mice genetically selected for maximal or minimal inflammatory reactions by means of histomorphometric characterization. After surgery in the experimental group will be induced dry socket by a suspension of purulent secretion, cotaining microorganisms associated with human alveolites. The samples will be submitted to histological processing and quantitative and qualitative histomorphometric microscopic analyses will be performed. The data will be statistically analyzed and compared between different strains, groups and time points. Since the initial proposal stage for the first phase of this project result in interesting data regarding the expected modulation, a second stage (any application for renewal of the grant / project) will include molecular analysis to study the possible mechanisms involved in modulation. The joint analysis of these data certainly will contribute to improve the understanding of the alveolar bone repair process, promoting specific knowledge that can cooperate significantly to the development of new strategies for diagnosis, prevention and therapy of bone diseases.