The aim of this study was to evaluate the tissue response to implanted polyethylene tubes filled with fibrin sponge embedded with silver nanoparticles dispersion. Thirty rats will individually receive four polyethylene tubes, two filled with sponge embedded in 47ppm or 23ppm silver nanoparticles dispersion, one filled with fibrin sponge embedded with 2,5% sodium hypochlorite and one more empty tube as control. The observation periods will be 7, 15, 30, 60 e 90 days. After each period of time, six animals will be killed and the tubes and surrounding tissue removed, fixed and prepared to be analyzed in light microscope with glycol methacrylate embedding, 3 mm serial cutting and HE staining. Scores will be defined as follows: 0, none or few inflammatory cells, no reaction; 1, <25 cells, mild reaction; 2, 25 to 125 cells, moderate reaction; 3, >125 cells, severe reaction. Fibrous capsule was categorized as "thin" when thickness was < 150 ¼m and "thick" at > 150 ¼m. Necrosis and formation of calcification were recorded. The data will be statistically analyzed using Kruskal Wallis test.
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