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"Effects of Portland cement pure and associated with two radiopacifying agents over cellular proliferation of fibroblasts."

Grant number: 09/11585-4
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): October 01, 2009
Effective date (End): November 30, 2010
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Marco Antonio Hungaro Duarte
Grantee:Camila Slompo
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


The aim of the present project will be to evaluate the behaviorof fibroblasts NiH-3T3 line submitted the different cementendodontics types (MTA Angelus Branco, Portland cement withzirconium oxide and Portland pure cement) and concentration. CellsNIH-3T3 will be incubated with different extract concentration(increasing dilutions: pure, 1/10, 1/100 e 1/1000) at 37oC, at 12, 24and 48 hour. The conditional and fibroblasts ways will be collectedand evaluated how much to the cellular viability (reduction of theMTT, Neutral Red capture and Crystal Violet staining). The statisticalanalyses will be made through the tests ANOVA, and have tested "t",in accordance with its indication (Turkey's test if p<0.05).

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CAMILA SLOMPO; CAMILA PERES-BUZALAF; KELLEN CRISTINA DA SILVA GASQUE; CARLA ANDREOTTI DAMANTE; RONALD ORDINOLA-ZAPATA; MARCO ANTONIO HUNGARO DUARTE; RODRIGO CARDOSO DE OLIVEIRA. Experimental Calcium Silicate-Based Cement with and without Zirconium Oxide Modulates Fibroblasts Viability. Brazilian Dental Journal, v. 26, n. 6, p. 587-591, . (09/11585-4)

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