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Diagnosis of Neospora caninum in bovine fetuses by hemi-nested PCR

Grant number: 05/00903-4
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2005
Effective date (End): December 31, 2006
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Principal Investigator:Claudia Del Fava
Grantee:Kelly Cristina Santos Montanari
Host Institution: Instituto Biológico (IB). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil


Neospora caninum is a protozoan that causes abortion and infertility in cows, stillbirth and encephalites in calves. In Brazil, seroepidemiological studies indicate that this parasite is disseminated in herds around the country. There are few publications about the isolation and identification of this parasite. The diferencial diagnose of bovine foetal losses should include neosporosis. The hemi-nested PCR has a hight sensibility and specificity to detect the DNA of Neospora caninum. It will be studied the presence of Neospora caninum by hemi-nested PCR in bovine foetuses from different regions of Brazil.

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