The commercial unavailability of chemical reference compounds for Brazilian plant species of medicinal interest represents a great barrier for the phytoterapic development, because it makes unfeasible the extracts standardization and its quality control. Phyllanthus, known popularly as quebra-pedra, it is used for years in the popular medicine and its potentiality as phytotherapic has been proven for scientific researches. Among the Phyllanthus species presented in Brazilian Pharmacopoeia meet the P. niruri and the P. tenellus, however the inclusion of the P. amarus still was not made, probably for the lack of standards and analytical methods that characterize this species. Considering the important pharmacological activities of the lignans present in P. amarus the analytical method development by high performance liquid chromatography will contribute to the standardization of active plante extracts and its quality control, differentiating each species. For the analytical development, it becomes necessary the isolation from lignans fractions and purification of the lignans unavailable commercially, representing one of the goals to be reached in this work. As form of guaranteeing the results quality obtained in the lignans (hipofilantina, filantina, nirtetralina and nirantina) quantification presents in the plant whole or extracts of P. amarus, the developed method will be validated properly according to the norms of the Agência de Vigilância Sanitária - ANVISA.
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