Glial cells are emerging from the background to become more prominent in our thinking about integration in the nervous system. Recently, these cells are seen as integrating neurons inputs and modulate synaptic activity. Astrocytes surround many of the synaptic connections in the central nervous system. The possible roles for this intimate association are not clear. Our objective will be examine whether estradiol or SERMs can induce change in astrocytes shape. To this end, we will study the presence of GFAP in slices of cortex from human temporal lobe applying immunoperoxidase and molecular techniques. Histologically normal temporal lobe cortex will be obtained from discarded tissue during surgery from intractable epilepsy, immediately sliced using a vibratome (400 µm) and cultured with 17β-estradiol-supplemented media (10-8M), raloxifen (10-6M), tamoxifen (10-6M) or control medium for 60 min. The materials were studied by immunohistochemistry for GFAP (anti-GFAP).
News published in Agência FAPESP Newsletter about the scholarship: