Study of ruthenium carboxylates of general formula [Ru2O(carb)2(py)6]n (carb = ibuprofen and ketoprofen): structural characterization and study of its in vitro metabolism.

• Grant number: 10/02717-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): May 01, 2010
• Effective date (End): April 30, 2011
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Inorganic Chemistry
• Principal Investigator: Sofia Nikolaou
• Grantee: Gabriela de Campos Seuanes
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

This project proposes the study of two novel ruthenium complexes with potential application as metallo-drugs. In this context, we intend to investigate the complexes [Ru2O(ibu)2(py)6](PF6)2 and [Ru2O(keto)2(py)6](PF6)2, where ibu and keto are the nonsteroidal anti-inflammatory drugs (NSAIDs) ibuprofen and ketoprofen. This proposal is a continuation of a work previously performed by the student (PIBIC fellowship, under my supervision), who has developed synthetic routes for the ruthenium complexes with mu-oxo bridge and the carboxylates of interest. Also, it was studied the purification of complexes by adsorption chromatography and they were characterized by the techniques of absorption spectroscopy in the UV-visible, cyclic voltammetry and differential pulse voltammetry and evaluated the behavior of the complexes in solutions of different pHs. Due to the good results obtained so far, the good performance of the student and the end of her fellowship, we intend to continue this working through this request to FAPESP. Thus, in the context above, the specific objectives of this proposal are: - Complement the characterization of the complexes through the techniques of mass spectrometry, nuclear magnetic resonance and infrared spectroscopies, and carry out the study of metabolism in vitro by high performance liquid chromatography (HPLC) of the two complexes obtained. For that, it will be used to microssomal fraction isolated from rats livers. This research project will be conducted in collaborative work with Prof. Anderson Rodrigo de Oliveira Moraes, Department of Chemistry FFCLRP, an expert in the separation techniques HPLC and capillary electrophoresis and already has approval from the Ethics Committee of the unit for the use of rats.