Influence of flavonoids against oxidative stress in children submitted to cardiac surgery

• Grant number: 06/03079-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2007
• End date: January 31, 2008
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Ulisses Alexandre Croti
• Grantee: Rafael Clark de Oliveira Piteri
• Host Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil

Abstract

The cardiopulmonary bypass (CPB) is regarded as an important cause of a complex inflammatory reply, which contributes significantly to several postoperative complications, such as renal, pulmonary and neurological problems, hemorrhages and even multiple organ dysfunction. This is due to the process of isquemia and reperfusion, to which the heart is submitted, and the production of free radicals that comes from this process. Flavonoids are antioxidants poliphenols that naturally exist in vegetables, fruits and beverages. The antioxidant action of these composites occurs through the removal of superoxide radicals, oxygen singlet and lipids peroxides, by means of the inhibition of the oxidation of lipoproteins of low density (LDL), the inhibition of the platelet aggregation, the reduction of the cellular proliferation and modulation of vascular tonus. 30 individuals will be studied divided to three groups - 10 patients with interatrial communication (IAC) who will be submitted to the cardiopulmonary bypass (CPB) and will receive grape juice; Group B - 10 patients with IAC who will be submitted to the CPB and will receive placebo and Group C - 10 patients with persistence of ductus arteriosus who won’t be submitted to CEC and will not receive either grape juice or placebo. Peripheral blood will be extracted for the dosage of malondyhaldeide, nitrites, nitrates, uric acid and total antioxidant capacity. Moreover, flavonoid levels will be dosed in the grape juice and in the patients. The quantitative variables with gaussian distribution will be analyzed through t test or the analysis of variance. The quantitative variables with no gaussian distribution will be analyzed by the application of the test of Mann-Whitney or Kruskal-Wallis. For the intergroup either the paired t test or Wilcoxon will be used, according to the need. An alfa error of 5% will be accepted, with a level of significance up to p<0,05. Keywords: Flavonoids, Oxidative Stress, Pediatric Cardiac Surgery (AU)