Screening of plants with anticariogenic properties from Brazilian Pantanal

• Grant number: 08/04114-2
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): January 01, 2009
• Effective date (End): November 30, 2011
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal researcher: Cristiane Yumi Koga Ito
• Grantee: Fernanda Lourenção Brighenti
• Home Institution: Faculdade de Odontologia (FOSJC). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

In the past decades, the interest on natural products has increased. The aim of this study is to evaluate plants extracts from the Brazilian Pantanal regarding their antimicrobial and citotoxicity in order to identify plants that show potential anticariogenic properties. The extracts will be obtained using leaves harvested in different seasons and using two solvents (water or alcohol). The following microrganisms will be employed: Actinomyces naeslundii ATCC 19039, Candida albicans ATCC 18804, Lactobacillus acidophilus 4356, Streptococcus gordonii ATCC 10558, S. mitis ATCC 9811, S. mutans ATCC 35688, S. sanguinis ATCC 10556 e S. sobrinus ATCC 33478. On Working Package 1, the active extracts will be identified by the agar diffusion test and will be tested regarding their maximum inhibitory dilution. The extracts with the highest activity will be evaluated regarding: a) mass recovery; b) stability; c) chemical caracterization by thin layer chromatography. The extracts with the best results will be studyed regarding their kinetics of antimicrobial activity in suspension and in biofilm and influence of sub-inhibitory concentrations on microorganisms adherence to glass. On Working Package 2, the extract that presented the highest antimicrobial activity will be evaluated regarding their citotoxicity in vitro (analysis of mitocondrial activity from human epithelial cells) and in vivo (histopathological evaluation of rats mucosa and internal organs). The more active extracts and less citotoxic will be finally evaluated in a model of microbial interaction between S. mutans and C. albicans that will be developed.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRIGHENTI, FERNANDA LOURENCAO; SALVADOR, MARCOS JOSE; LACERDA GONTIJO, ALINE VIDAL; BOTAZZO DELBEM, ALBERTO CARLOS; BOTAZZO DELBEM, ADINA CLEIA; SOARES, CRISTINA PACHECO; CARVALHO DE OLIVEIRA, MARIA ALCIONEIA; GIRONDI, CAMILA MIORELLI; KOGA-ITO, CRISTIANE YUMI. Plant extracts: initial screening, identification of bioactive compounds and effect against Candida albicans biofilms. FUTURE MICROBIOLOGY, v. 12, n. 1, p. 15-27, JAN 2017. (08/04114-2, 14/09666-4)

BRIGHENTI, F. L.; SALVADOR, M. J.; BOTAZZO DELBEM, ALBERTO CARLOS; BOTTAZZO DELBEM, ADINA CLEIA; OLIVEIRA, M. A. C.; SOARES, C. P.; FREITAS, L. S. F.; KOGA-ITO, C. Y.. Systematic Screening of Plant Extracts from the Brazilian Pantanal with Antimicrobial Activity against Bacteria with Cariogenic Relevance. Caries Research, v. 48, n. 5, p. 353-360, 2014. (08/04114-2)