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Efeitos das estatinas sobre a viabilidade bacteriana e sobre o biofilme de Staphylococcus aureus

Author(s):
Talita Signoreti Graziano
Total Authors: 1
Document type: Master's Dissertation
Institution: Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba
Defense date:
Examining board members:
Cristiane de Cássia Bergamaschi; Pedro Luiz Rosalen
Advisor: Karina Cogo Müller
Abstract

Statins are drugs that competitively inhibit the enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA). Besides their important lipid-lowering action, they also are pleiotropic agents. Several studies have explored a possible protective effect of statins to reduce the morbidity and mortality of various infectious diseases. The antimicrobial activity of statins has been reported by in vivo and in vitro studies. The aim of this study was to evaluate the effects of statins on the growth, viability and biofilm formation of pathogenic aerobic bacteria. The Minimum Inhibitory Concentrations (MIC) of atorvastatin, pravastatin and simvastatin against planktonic cells of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus faecalis strains were obtained. Since simvastatin showed activity against S. aureus, its effects on cell viability were evaluated in a time-kill and post-antibiotic effect (PAE) assays. A possible synergistic effect between simvastatin and vancomycin was also assessed. In addition, the effect of simvastatin against biofilms of S. aureus was tested. The MIC values of simvastatin for S. aureus were: 15.65 µg/ml (ATCC 29213) and 31.25 µg/ml (ATCC 33591, 43300, 14458 and 6538). Simvastatin showed a bacteriostatic profile, and in a 4x>MIC concentration the PAE was similar to vancomycin. No synergistic effect was found between simvastatin and vancomycin. Simvastatin was able to reduce the formation of biofilms in concentrations ranging from 1/8MIC to 4xMIC. In addition, the 4xMIC was able to decrease the viability, biomass and production of extracellular polysaccharides and increase the production of intracellular polysaccharides on mature biofilm of S. aureus. The protein production on biofilm was not altered in the presence of simvastatin . In conclusion, our results showed that simvastatin has a great potential to be explored, especially in relation to the development new antimicrobial agentes. (AU)

FAPESP's process: 11/15795-3 - ANALYSIS OF THE EFFECTS OF STATINS ON THE BACTERIAL VIABILITY AND BIOFILM FORMATION OF PATHOGENIC AEROBIC MICROORGANISMS
Grantee:Talita Signoreti Graziano
Support type: Scholarships in Brazil - Master