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| Author(s): |
Josy Goldoni Lazarini
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Piracicaba, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba |
| Defense date: | 2016-02-23 |
| Examining board members: |
Pedro Luiz Rosalen;
Patricia Correa Dias;
Bruno Bueno Silva
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| Advisor: | Pedro Luiz Rosalen |
| Abstract | |
In the search for alternative bioactive molecules, Brazilian native fruit species have demonstrated functional potential due to the presence of phenolic compounds such as flavonoids, anthocyanins and others in their composition. Previous studies performed by our research group found that parts (leaves, seeds and pulps) of the Eugenia species (E. brasiliensis, E. uniflora, E. leitonii e E. myrcianthes have antioxidant and anti-inflammatory activity. In adition the seeds of E. leitonii showed better activity in decrease of neutrophil influx into the peritoneal cavity of mice. Thus, continuing the investigation of unexplored Brazillian native fruits, this study evaluated the anti-inflammatory activity, mechanism(s) of action and phytochemical profile of the extract from the seeds of E. leitonii. The hydroethanolic extract (80:20, v/v) of E. leitonii seeds (EL) was evaluated by means of tests assessing cytotoxicity, modulation of inflammatory mediators (TNF-?, IL-1? and CXCL2/MIP-2) and NF-?B activation in RAW 264.7 macrophage cells. The EL extract was assess in vivo concerns the inflammatory mediators levels (TNF- ?, IL-1? and CXCL2/MIP-2) in the peritoneal fluid of the animals; the influx of neutrophil migration into their peritoneal cavity; evaluation of ICAM-1 and P¿selectin expression in vascular endothelium; and carrageenan-induced paw edema. Finally, the total phenolic content of the extract was measured and phenolic compounds were identified by LC-MS/MS. The results showed that EL extract did not affect significantly (P > 0.05) cell viability at the concentrations ranging from 0.02 to 200 mg µl-1; at 200 mg µl-1 reduced the levels of TNF-? and CXCL2/MIP-2, and NF-kB activation in RAW 264.7 cells (P < 0.05). Mice pre-treated orally with EL extract (300 mg kg-1 ) showed significantly decreased levels of TNF-? and CXCL2/MIP-2, also there was a decrease in ICAM-1 and P¿selectin expression as compared with the control group (P < 0.05). The mice pretreated at doses 30, 100 e 300 mg kg-1 showed decrease of the influx of neutrophils into the peritoneal cavity of the mice as compared with the negative control group carrageenan (P < 0.05) and finally, the mice pretreated orally with EL extract at 300 mg kg-1 demonstrated maximum reduction of edema at 3 h (64 %) when compared with carrageenan (P < 0.05). The EL extract showed total phenolic contents yielded 158.74 ± 3.5 mg GAE/g and flavonoids and anthocianins were identified by LC-MS/MS. Thus, this genuine Brazilian species founded in Atlantic Forest, an ecosystem threatened, showed to be a promising source of bioactive compounds with anti-inflammatory potential, acting on pathways of inflammatory mediators involving rolling and adhesion molecules and showed anti-edematogenic effect in the paw edema. The EL extract exhibited content of phenolic compounds (flavonoids and anthocyanins) higher than that of traditional fruits, which may be related with the anti-inflamatory effects observed allowing it use to enriching foods (AU) | |
| FAPESP's process: | 13/26251-0 - Prospection of anti-inflammatory activity in native fruit species from Brazil |
| Grantee: | Josy Goldoni Lazarini |
| Support Opportunities: | Scholarships in Brazil - Master |