Characterization and evaluation of the role of wcbE gene from Burkholderia seminalis strain TC3.4.2R3 in microbial interaction.

Author(s)

Priscila Jane Romano de Oliveira Gonçalves

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2017-06-26

Examining board members

Welington Luiz de Araujo; Fernando Dini Andreote; Rodrigo da Silva Galhardo; Luciane Maria Pereira Passaglia; Ana Olívia de Souza

• Advisor: Welington Luiz de Araujo

Abstract

Burkholderia seminalis has been found in both pathogenic and nonpathogenic interactions. The wcbE gene encodes a glycosyltransferase and belongs to the wcb cluster, which is related to capsule synthesis. The aim of this work was to investigate the role of the wcbE gene and temperature in the microbial interactions of B. seminalis TC3.4.2R3. The production of biofilm, EPS and antifungal compounds was higher at 28 ºC. On the other hand, the motility, virulence and stress responses were higher at 37 ° C. wcbE produced less biofilm than WT and was attenuated in G. mellonella at 37 ° C, highlighting the importance of glycosyltransferase in the pathogenesis. Furthermore, wcbE lost the ability to inhibit phytopathogenic fungi. Although B. seminalis is a member of Bcc, it is effective against clinical and environmental pathogens, indicating that this strain may have multiple interactions in the environment. The temperature and the glycosyltransferase gene played a crucial role in the environmental interactions of B. seminalis TC3.4.2R3. (AU)

• FAPESP's process: 13/03426-9 - Characterization of wcb cluster of B. seminalis strains TC3.4.2R3 and its role in the biosynthesis of antimicrobial compounds
• Grantee: Priscila Jane Romano de Oliveira Gonçalves
• Support Opportunities: Scholarships in Brazil - Doctorate