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| Author(s): |
Maria Alice Garcia Bento
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Jaboticabal. 2018-04-25. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal |
| Defense date: | 2018-03-26 |
| Advisor: | Fernando José Zara |
| Abstract | |
The Dromiidae family is considered basal within Brachyura, included in the section Podotremata Guinot, 1977 and is poorly known from histo-morphological and ultrastructural point of view. This dissertation shows the sperm ultrastructure and the anatomo-histological patterns of the reproductive system to the Brazilian dromiid species. In addition, we studied the mating behavior and spermathecal morphology in Hypoconcha parasitica to elucidate some points of the fertilization in dromiid crabs. The samples were processed according to the routine for histology and histochemistry and also, to transmission and scanning electron microscopy. There are no distinct ultrastructural characters to sperm ultrastructure among Dromioidea. Additionally, only specific characters were observed for studied Dromiidae as the presence of the spherical flange in the anterolateral eletron-lucid zone and the granular acrosomal outer zone founded in H. parasitica, absence of the acrosome ray zone and presence of the flange remnants in Moreiradromia antillensis. The perforatorial chambers show specific morphology among all species studied. The Dromiidae spermatozoa ultrastructure were not consistent to separate Dromiidae from the other Dromioidea. The ultrastructural differences were only found when compared Dromiodea with other Podotremata as Homolidae, Latreillidae e Raninidae. The seminal fluid production and spermatozoa package in the vas deferens of Dromiidae are totally different from Eubrachyura. In H. parasitica, Hypoconcha arcuata, M. antillensis and Dromia erythropus, the spermatophores are absent, and they show only a long internal spermatic cord surrounded by secretions in vas deferens, which is similar to the species of Astacidae. The mating behavior is simple without courtship and the females copulated in both soft and hard during moulting condition. The storage of seminal material inside the spermatheca is distinct from Eubrachyura. The morpho-histological organization of the spermathecae indicates that the process of sperm release for fertilization occurs through muscular contractions of its wall, and the pleopod 1 may promote the oocytes and spermatozoa movement to ensure the fertilization. (AU) | |
| FAPESP's process: | 16/10394-4 - Male reproductive system and espermiotaxonomy in Dromiidae: there is only one pattern of seminal fluid production, packaging sperm and sperm transfer in crabs? |
| Grantee: | Maria Alice Garcia Bento |
| Support Opportunities: | Scholarships in Brazil - Master |