Obtaining of alternative prebiotic sources and its use in broiler diets

Author(s)

Mayara Rodrigues Pivetta

• Document type: Doctoral Thesis
• Press: Botucatu. 2016-06-30.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu
• Defense date: 2016-06-03
• Advisor: Luciana Francisco Fleuri; Antonio Celso Pezzato

Abstract

Inulinases catalyze the inulin hydrolysis, yielding inulo-oligosaccharides, fructose and glucose, therefore, they have many industrial applications. The submerged fermentation is more used at inulinase industrial production, however the solid state fermentation (SSF) has showed good results regarding to low cost enzymes. The yacon (Smallanthus sonchifolius) is a tuber root with high content of antioxidants and fructans and because of this it has attracted interest of food area. The solid state fermentation using the yacon root as substrate has potential to produce enzymes and to use in animal feeding. The aim of this work was to evaluate the inulinase production of 16 filamentous fungal species growing in solid state fermentation (SSF) on agroindustrial wastes as substrate, as well as to identify the best combination substrate/microorganism of the solid enzymatic preparation (SEP) to use as prebiotic in broilers diets. The inulinase activity was higher with yacon flour than wheat bran, soybean bran and sugarcane bagasse, independently of fungus. The inulinases from yacon flour showed large pH and temperature amplitude, independently of fungus. The SEP of yacon flour and Aspergillus niger 40018 showed high lipase and antioxidant activity and high fructooligosaccharides and it was selected to further trials with broilers. There was no alteration on performance, caecum pH and lipid profile with the SEP. The SEP promoted a stronger reduction of Salmonella Enteritidis number after the birds inoculation, and, consequently, the mucosal gut turnover was slower. It is concluded that the SEP of yacon flour with A. niger 40018 has prebiotic effect. (AU)

• FAPESP's process: 13/15481-4 - OBTAINMENT OF ALTERNATIVE PREBIOTIC SOURCES AND USE IN BROILER DIETS
• Grantee: Mayara Rodrigues Pivetta
• Support Opportunities: Scholarships in Brazil - Doctorate