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Bioprospecting and phylogeny of fluorescent bacteria of the genus Pseudomonas as potential biocontrol agents of the foliar blight of signal grass

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Author(s):
Samara Nunes Campos Vicentini
Total Authors: 1
Document type: Master's Dissertation
Press: Ilha Solteira. 2018-12-11.
Institution: Universidade Estadual Paulista (Unesp). Faculdade de Engenharia. Ilha Solteira
Defense date:
Advisor: Paulo Cezar Ceresini
Abstract

Biological control is one of the methods of the integrated management of plant diseases, which is one of the only viable alternative to minimizes foliar blight impact and sudden death of pastures from genus Urochloa (previously known as Brachiaria). Foliar blight and death of pastures are caused by the fungus Rhizoctonia solani AG-1 IA. In our study, it was determined whether isolates from florescent Pseudomonas obtained from suppressive soils on the Amazon biome had potential as biocontrol agents of Rhizoctonia solani AG-1 IA. Specifically, we aim to: i. Carry out a screening of isolates from Pseudomonas to evaluate their potential as in vitro biocontroler; ii. Perform molecular identification of selected fluorescent Pseudomonas isolates as potential biocontrol agents; and iii. Determine whether in vitro selected bacterial strains maintain the biocontrol ability in vivo of foliar blight and sudden death of braquiária. Three isolates were selected as potential biocontoller agents in vitro antagonism tests (Amana, Poti e Yara), which reduced significantly mycelial growth and sclerotia germination of pathogen. Based on phylogenetic analyzes, Amana and Yara isolates were grouped to the clade belonging to Pseudomonas putida and Poti isolated was grouped in a distinct clade, and should to constitute a new species. For in vivo tests, seed inoculation did not resulted in R. solani AG-1 IA control. On the other hand, in foliar application of biocontrol agent, Amana isolated was the most efficient phypathogen controller. (AU)

FAPESP's process: 17/03403-0 - Bioprospecting of fluorescent bacteria of the genus Pseudomonas in the biocontrol of the foliar blight of signal grass (Urochloa brizantha)
Grantee:Samara Nunes Campos Vicentini
Support type: Scholarships in Brazil - Master