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Studies on the production of antimycin and mannopeptimycin in Streptomyces sp. CBMAI 2043

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Ana Beatriz Gonçalves
Total Authors: 1
Document type: Master's Dissertation
Defense date:
Advisor: Luciana Gonzaga de Oliveira

Streptomyces are bacteria that inhabit predominantly the soil but the secondary metabolism of these microorganisms is extensively studied as it is rich in molecules with different applications in the pharmaceutical industry. An example of this vast metabolism and its functions can be recognized in the discovery of antibiotics in the twentieth century; these entities were mostly isolated from different strains of Streptomyces. Among the variety of metabolites produced by these microorganisms are antimycins, a respiratory chain inhibitor, which is used as a piscicide, and the mannopeptimycins, a glycopeptide, isolated primarily from S. hygroscopicus, that act against strains resistant to the most potent antibiotics, such as methylcyclines and vancomycin. In this sense, this project aimed to study the production of these metabolites, encoded in Streptomyces sp. CBMAI 2043 genome performing mutations in the wild strain and expressing genes clusters related to the biosynthesis of these metabolites in a heterologous strain (S. coelicolor) in order to expand the knowledge about the enzymatic machinery producing these molecules and possible analogues (AU)

FAPESP's process: 14/12727-5 - Genome mining in Streptomyces
Grantee:Luciana Gonzaga de Oliveira
Support type: Regular Research Grants